| 摘要: |
| 目的 研究乾坤丹Ⅵ号对糖尿病肾病（DN）的治疗作用及可能的机制。观察其对DN模型大鼠结缔组织生长因子（CTGF）、转化生长因子-β1（TGF-β1）、骨形态发生蛋白-7（BMP-7）的影响。方法 通过皮下注射链脲佐菌素（STZ）联合高糖高脂饮食以制备大鼠DN模型，成模大鼠随机分为模型组和治疗组，并设空白对照组，成模后给予药物治疗，给药12周后检测大鼠血糖，尿微量白蛋白，Western blot法检测肾脏CTGF、TGF-β1、BMP-7表达水平，逆转录-聚合酶链反应（RT-PCR）法检测CTGF、TGF-β1、BMP-7 mRNA转录水平。结果 与空白对照相比，模型组血糖、尿微量白蛋白显著升高，CTGF、TGF-β1蛋白表达和mRNA转录水平显著上调，BMP-7蛋白表达和mRNA转录水平显著下调；乾坤丹Ⅵ号治疗后血糖，尿微量白蛋白显著低于模型组，CTGF、TGF-β1蛋白表达和mRNA转录水平与模型组相比也显著降低，BMP-7蛋白表达和mRNA转录水平显著升高。结论 乾坤丹Ⅵ号对DN模型大鼠有治疗作用，其作用机制可能与调节TGF-β1-CTGF-BMP-7轴相关。 |
| 关键词: 糖尿病肾病 乾坤丹Ⅵ号 CTGF GF-β1 BMP-7 |
| DOI：10.11656/j.issn.1672-1519.2022.06.21 |
| 分类号:R587.2 |
| 基金项目:天津市科技计划项目（16ZXZYNC00060）；天津市北辰区卫健系统科技项目（SHGY-2020037） |
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| Effect and mechanism of Qiankun Dan Ⅵ on diabetic nephropathy model rats |
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LI Lianrui1, CHEN Zhiyong1, LIU Jie1, LI Tianxiang2
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1.Department of Pharmacy, Tianjin Beichen Hospitial of Traditional Chinese Medicine, Tianjin 300400, China;2.School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| Objective To investigate the therapeutic effect of Qiankundan Ⅵ on diabetic nephropathy (DN) and its possible mechanism, and to observe its effects on CTGF, TGF-β1 and BMP-7 in DN model rats.Methods By subcutaneous injection of STZ joint high sugar, high fat diet to the preparation of DN rats model, the model for the preparation of successful rats were randomly divided into model group and treatment group, and blank control group, giving medication, the dose after 12 weeks of detecting the blood sugar, the urine trace albumin, kidney CTGF, TGF-β1, BMP 7 expression level of rats by Western blot, the CTGF, TGF-β1, BMP 7 mRNA transcription level of rats by RT-PCR method.Results Compared with the blank group, blood glucose and urinary microalbumin in the model group were significantly increased, protein expression of CTGF and TGF-β1 and mRNA transcription were significantly up-regulated, protein expression of BMP-7 and mRNA transcription were significantly down-regulated. After treatment with Qiankun Dan Ⅵ, blood glucose, urinary trace albumin were significantly lower than those in the model group, protein expression and mRNA transcription level of CTGF and TGF-β1 were also significantly lower than those in the model group, and protein expression and mRNA transcription level of BMP-7 were significantly increased.Conclusion Qiankun Dan Ⅵ has therapeutic effects on DN rats, and its mechanism may be related to regulating TGF-β1-CTGF-BMP-7 axis. |
| Key words: diabetic nephropathy Qiankun Dan Ⅵ CTGF TGF-1β1 BMP-7 |
