宣肺化瘀通脉方对野百合碱诱导大鼠肺动脉高压的影响

Effect of Xuanfei Huayu Tongmai Decoction on rats with monocrotaline induced pulmonary hypertension

关键词:  肺动脉高压  宣肺化瘀通脉方  氧化应激

Key words:  pulmonary hypertension  Xuanfei Huayu Tongmai Decoction  oxidative stress

摘要:

摘要：[目的] 探讨宣肺化瘀通脉方对野百合碱（MCT）诱导的肺动脉高压（PH）大鼠氧化应激水平的影响。[方法] 将70只SPF级雄性SD大鼠，随机分为正常对照组（20只）和MCT组（50只）；MCT组单次腹腔注射MCT（60 mg/kg），诱导14 d建立PH模型。第15天对照组、MCT组各随机抽取10只，检测大鼠平均肺动脉压（mPAP）的变化。将造模成功后的40只大鼠随机分为模型组、西地那非组、宣肺化瘀通脉方低剂量组及高剂量组，每组10只，各用药组均灌胃给药，低剂量组为6.75 g生药/kg，高剂量组为13.5 g生药/kg，每日1次，连续给药14 d，对照组及模型组给予等容积蒸馏水。观察宣肺化瘀通脉方对PH大鼠mPAP、右心室收缩压（RVSP）、右心室肥厚指数（RVHI）、肺组织形态及氧化应激水平的影响。[结果] 宣肺化瘀通脉方可显著降低PH大鼠RVSP、mPAP及RVHI水平，降低肺部动脉管壁厚度、改善血管狭窄程度，改善PH肺组织病理损伤；降低PH大鼠血清活性氧（ROS）含量，上调环氧合酶1（COX-1）、环氧合酶2（COX-2）表达水平，升高过氧化氢酶（CAT）活性。[结论] 宣肺化瘀通脉方可显著改善MCT诱导的大鼠PH，其作用机制与调控氧化应激水平有关。

Abstract:

[Objective] To explore the effect of Xuanfei Huayu Tongmai Decoction treating oxidative stress level in rats with pulmonary hypertension (PH) induced by monocrotaline (MCT)).[Methods] The 70 SPF male SD rats were randomly divided into control group (20 rats) and MCT group (50 rats).In MCT group,a single intraperitoneal injection of MCT (60 mg/kg) was used to induce PH model for 14 days.On the 15th day,10 rats in control group and MCT group were randomly selected.The changes of mean pulmonary artery pressure (mPAP) in rats were detected.After successful modeling,40 rats were randomly divided into model group,sildenafil group,low-dose group and high-dose group of Xuanfei Huayu Tongmai Decoction.All groups were given intragastric administration once a day for 14 days and the low-dose group was 6.75 g crude drug/kg and the high-dose group was 13.5 g crude drug/kg,otherwise the control group and model group were given equal volume distilled water.To observe the effects of Xuanfei Huayu Tongmai Decoction on mPAP,right ventricular systolic pressure (RVSP),right ventricular hypertrophy index (RVHI),lung tissue morphology and oxidative stress level in PH rats.[Results] Xuanfei Huayu Tongmai Decoction can significantly reduce the levels of RVSP,mPAP and RVHI in PH rats,improve the thickness of pulmonary artery wall,improve the degree of vascular stenosis and inhibit the pathological damage of PH;Lowering the serum reactive oxygen species (ROS) content of PH rats,up-regulate the expression level of cyclooxygenase-1(COX-1),cyclooxygenase-2(COX-2) and the activity of catalase (CAT).[Conclusion] Xuanfei Huayu Tongmai Decoction can significantly improve the PH of rats induced by MCT,and its mechanism is related to regulating the level of oxidative stress.