| 摘要: |
| [目的] 利用代谢组学方法探究注射用丹参多酚酸治疗缺血性脑卒中恢复期的作用机制。[方法] 采用超高效液相色谱质谱联用仪（UHPLC-Q-Orbitrap MS）对39例符合相应纳入、排除标准的缺血性脑卒中恢复期患者的血液样本进行代谢组学检测，通过无监督的主成分分析（PCA）、有监督模式的正交偏最小二乘法判别分析（OPLS-DA）对患者用药治疗前后的血浆进行分析。[结果] 代谢组学共筛选出42个与治疗疾病相关的生物标记物，涉及22种代谢通路，其中磷脂生物合成、磷脂酰胆碱生物合成、卟啉代谢、组氨酸代谢、类固醇生成、嘧啶代谢为变化最显著的6条代谢通路。[结论] 通过代谢组学方法证实，注射用丹参多酚酸可能通过调节磷脂代谢、氨基酸代谢、核酸代谢及能量代谢，进而发挥治疗缺血性脑卒中恢复期的作用。表明代谢组学对指导中医临床及机制研究方面的可行性。 |
| 关键词: 缺血性脑卒中 注射用丹参多酚酸 代谢组学 |
| DOI：10.11656/j.issn.1672-1519.2022.12.02 |
| 分类号:R285.5 |
| 基金项目:国家重点研发计划项目(2018YFC1707404)。 |
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| Investigation on the mechanism of salvianolic acid for injection in the treatment of ischemic stroke based on metabolomics |
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WANG Jingyang1, HAN Lifeng2, ZHANG Chen3, ZHAO Yanan3, XU Qiang3
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1.Postgraduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;3.Department of Cardiovascular Medicine, Second Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China
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| Abstract: |
| [Objective] To explore the mechanism of salvianolic acid for injection(SAI) in the treatment of ischemic stroke by metabolomics method. [Methods] UHPL-Q-Orbitrap MS was utilized to fulfill metabolomics in 39 patients' blood samples with ischemic stroke who met the corresponding inclusion and exclusion criteria in convalescent period,the plasma of patients before and after treatment of SAI was analyzed by unsupervised principal component analysis(PCA),and supervised orthogonal partial least squares discriminant analysis(OPLS-DA). [Results] A total of 42 biomarkers related to the treatment of ischemic stroke were screened out by metabolomics analysis,which involved 22 metabolic pathways. The significantly changed six metabolic pathways mainly included phospholipid biosynthesis,phosphatidylcholine biosynthesis,porphyrin metabolism,histidine metabolism,steroidogenesis,and pyrimidine metabolism. [Conclusion] Confirmed by metabolomic approach,the mechanism of SAI on the treatment of ischemic stroke was possibly related to the regulation of phospholipid metabolism,amino acid metabolism,nucleic acid metabolism and energy metabolism. And it is indicated that metabonomics is feasible to guide the clinical and mechanism research of traditional Chinese medicine. |
| Key words: ischemic stroke salvianolic acid for injection metabolomics |
