超高效液相色谱法同时测定川木香中紫丁香苷、木香烃内酯和去氢木香内酯含量

Simultaneous determination of syringin，costunolide and dehydrocostus lactone in vladimiriae radix by UPLC

关键词:  川木香  微波提取  UPLC  含量测定

Key words:  vladimiriae radix  microwave extraction  UPLC  content determination

摘要:

[目的] 采用微波提取，超高效液相色谱（UPLC）分析，建立同时对川木香中紫丁香苷、木香烃内酯、去氢木香内酯进行含量测定的方法。[方法] 以紫丁香苷、木香烃内酯、去氢木香内酯的提取率为评价指标，优化川木香的最佳微波提取条件，并采用UPLC对22批不同批次的川木香进行含量测定。[结果] 微波提取川木香的最佳条件为：提取溶剂为甲醇，微波功率400W，提取时间2min，液料比为80∶1（mL/g），在此条件下川木香中3种目标化合物的提取率均比超声提取法提取率高；采用UPLC建立的同时测定川木香中紫丁香苷、木香烃内酯、去氢木香内酯含量的方法线性关系良好，精密度、重复性、稳定性的RSD均小于3%，加样回收率介于范围101.79%~102.23%；含量测定结果表明，不同批次的川木香药材中各成分的含量差异较大，表明市场上的川木香药材质量有较大差异。[结论] 实验建立的方法稳定、快速、高效、重现性好，可用于川木香药材的质量评价。

Abstract:

[Objective] To establish a method for simultaneous determination the content of syringin,costunolide and dehydrocostus lactone in vladimiriae radix by microwave extraction with UPLC analysis. [Methods] The best microwave extraction process of vladimiriae radix was optimized by taking the extraction rates of syringin,costunolide and dehydrocostus lactone as indexes,and twenty-two batches of vladimiriae radix were determined content by UPLC. [Results] The optimum microwave extraction conditions of vladimiriae radix as follows:methanol as solvent,microwave power 400 W,extraction time 2 min,and the liquid/solid ratio of 80:1 (mL/g). The extraction rates of three target compounds in different batches of vladimiriae radix under these conditions were higher than those by ultrasonic extraction. The method used to determine simultaneously the content of syringin,costunolide and dehydrocostus lactone in vladimiriae radix by UPLC showed good linearity,the RSD of the precision,repeatability and stability tests were less than 3% and the average recoveries were ranged in 101.79%~102.23%. The results showed that the content of three compounds in vladimiriae radix of different batches were different. It showed that there were large differences in the quality of vladimiriae radix on the market. [Conclusion] The method established in this experiment was stable,rapid and efficient with good repeatability,which could be used for the quality evaluation of vladimiriae radix.