| 摘要: |
| [目的]观察痛泻要方合四逆散对KK-Ay小鼠胰岛素抵抗及磷脂酰肌醇3-激酶/蛋白激酶B/糖原合成酶激酶-3β（PI3K/AKT/GSK-3β）信号通路的影响。[方法]采用雄性6~8周龄KK-Ay小鼠建立糖尿病模型，随机分为模型组、中药低中高不同剂量组，另取同周龄C57 BL/6J小鼠作为正常组，干预4周后，检测小鼠空腹血糖，空腹胰岛素水平及胰岛功能情况，并通过实时荧光定量反转录聚合酶链式反应（real time PCR），蛋白免疫印迹（Western Blot）等方法检测小鼠肝脏中PI3K、AKT、GSK-3β的基因及蛋白磷酸化水平表达情况。[结果]与正常组相比，模型组小鼠空腹血糖和胰岛素抵抗指数明显升高（P＜0.05），而PI3K、AKT、GSK-3β的磷酸化蛋白水平及基因表达水平均明显降低（P＜0.05）；与模型组相比，中药高剂量组空腹血糖和胰岛素抵抗指数明显降低（P＜0.05），而PI3K、AKT、GSK-3β的磷酸化蛋白水平及基因表达水平均明显升高（P＜0.05）。[结论]痛泻药方合四逆散可能通过影响PI3K/AKT/GSK-3β信号通路改善KK-Ay小鼠的胰岛素抵抗。 |
| 关键词: 痛泻药方 四逆散 胰岛素抵抗 PI3K/AKT/GSK-3β信号通路 |
| DOI：10.11656/j.issn.1672-1519.2023.02.15 |
| 分类号:R587.1 |
| 基金项目:北京中医药大学青年教师项目（2018-JYBZZ-JS176）。作者简介：胡浩（1982-），女，硕士，主治医师，主要研究方向为中医药防治糖尿病及其并发症的临床和基础研究。 |
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| Effects of Tongxie Yaofang and Sini Powder on PI3K/AKT/GSK-3β signal pathway and insulin resistance in KK-Ay mice |
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HU Hao1, JIA Xiaolei2
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1.Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China;2.The Third Affiliated Hospital of Beijing University of Traditional Chinese Medicine, Beijing 100029, China
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| Abstract: |
| [Objective] To observe the effects of Tongxie Yaofang and Sini Powder on PI3K/AKT/GSK-3β signal pathway and insulin resistance in KK-Ay mice.[Methods] Male 6 to 8 week old KK-Ay mice were used to establish a diabetes model. They were randomly divided into model group,and low,medium,high dosage groups of Chinese medicine. C57BL/6J mice of the same week were taken as the normal group. After 4 weeks of intervention,fasting insulin levels,insulin and islet function of mice were detected,and the protein phosphorylation and mRNA expression of PI3K,AKT and GSK-3β in mice liver were detected by real time PCR and Western blot.[Results] Compared with the normal group,the fasting blood glucose and insulin resistance index of the model group mice were significantly increased (P＜0.05),while the phosphorylated protein levels and gene expression levels of PI3K,AKT,GSK-3β were significantly reduced (P＜0.05). Compared with the model group,the fasting blood glucose and insulin resistance index in the high dose group were significantly reduced (P＜0.05),while the phosphorylated protein levels and gene expression levels of PI3K,AKT,GSK-3β were significantly increased (P＜0.05).[Conclusion] Tongxie Yaofang and Sini Powder may improve insulin resistance in KK-Ay mice by affecting PI3K/AKT/GSK-3β signaling pathway. |
| Key words: Tongxie Yaofang Sini Powder insulin resistance PI3K/AKT/GSK-3β signaling pathway |
