| 摘要: |
| [目的]探究藏红花素(CRO)对幼年小鼠肺炎模型的治疗作用及其与丝裂原活化蛋白激酶(MAPK)/核因子-κB(NF-κB)通路的关系。[方法]采用腹腔注射脂多糖(LPS)构建幼年小鼠肺炎模型,将小鼠随机分为对照组、模型组、DEX(阳性对照-地塞米松)组、CRO低剂量组、CRO中剂量组、CRO高剂量组、MAPK激活剂(Anisomycin)组(CRO高剂量+Anisomycin)。检测小鼠肺湿/干(W/D)比和髓过氧化物酶(MPO)活性,苏木精-伊红(HE)染色观察肺部组织病理变化并进行病理损伤评分,血细胞计数器和吉姆萨染色进行总细胞、中性粒细胞和白细胞计数,酶联免疫吸附(ELISA)法检测血清和肺泡灌洗液(BALF)中炎性细胞因子水平,试剂盒检测血清和BALF中丙二醛(MDA)、超氧化物歧化酶(SOD),蛋白免疫印迹(Western Blot)检测MAPK/NF-κB通路蛋白表达。[结果]与模型组比较,DEX组和CRO低、中、高剂量组肺组织损伤减轻,W/D比、MPO活性、病理损伤评分、总细胞、中性粒细胞、白细胞计数、白介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)、MDA、p-p38 MAPK/p38 MAPK、p-NF-κB p65/NF-κB p65、p-IκBα/IκBα显著下降,SOD显著升高(P＜0.05);而Anisomycin可逆转高剂量CRO对肺炎小鼠的治疗作用(P＜0.05)。[结论] CRO对幼年肺炎小鼠有治疗作用,其作用机制可能与抑制MAPK/NF-κB通路有关。 |
| 关键词: 藏红花素 幼年小鼠 肺炎 MAPK/NF-κB通路 |
| DOI：10.11656/j.issn.1672-1519.2023.12.17 |
| 分类号:R563.1 |
| 基金项目:山东省中医药科技发展计划项目(2019-0043)。 |
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| Exploring the therapeutic effect and molecular mechanism of crocin on a young mouse pneumonia model based on the MAPK/NF-κB pathway |
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DONG Lijun1, ZHOU Bo2, YANG Cuiling3, LI Zhiyuan4, ZHANG Yongfeng5
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1.School of Clinical Medicine, Weifang Medical College, Weifang 261053, China;2.Department of Imaging, Anqiu People's Hospital, Anqiu 262199, China;3.Department of Pediatrics, Anqiu People's Hospital, Anqiu 262199, China;4.School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;5.Department of Pediatrics, Affiliated Hospital of Weifang Medical College, Weifang 261035, China
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| Abstract: |
| [Objective] To explore the therapeutic effect of crocin(CRO) on pneumonia in young mice and its relationship with mitogen activated protein kinase(MAPK)/nuclear factor-κB(NF-κB) pathway. [Methods] A young mouse pneumonia model was constructed by intraperitoneal injection of lipopolysaccharide(LPS). The mice were randomly grouped into control group,model group,DEX(positive control dexamethasone) group,CRO low-dose group,CRO medium-dose group,CRO high-dose group,and MAPK activator(Anisomycin group,CRO high-dose+Anisomycin). The wet to dry(W/D) ratio and myeloperoxidase(MPO) activity of mouse lungs were detected.Hematoxylin eosin(HE) staining was applied to observe pathological changes in lung tissue and pathological injury score was performed.Blood cell counters and Giemsa staining were applied to perform total cell,neutrophil,and white blood cell counts. Enzyme-linked immunosorbent assay(ELISA) was applied to detect levels of inflammatory cytokines in serum and alveolar lavage fluid(BALF). Kits were applied to detect malondialdehyde(MDA) and superoxide dismutase(SOD) in serum and BALF. Western blot was applied to detect the expression of MAPK/NF-κB pathway protein. [Results] Compared with the model group,the DEX group and CRO low,medium,and high dose groups showed reduced lung tissue injury,the W/D ratio,MPO activity,pathological injury score,total cells,neutrophils,white blood cell count,IL-1β,IL-6,TNF-α,MDA,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65,p-IκBα/IκBα were obviously decreased,SOD was obviously increased(P＜0.05). Anisomycin was able to reverse the therapeutic effect of high-dose CRO on pneumonia in mice(P＜0.05). [Conclusion] CRO has therapeutic effects on young pneumonia mice,and its mechanism may be related to inhibiting the MAPK/NF-κB pathway. |
| Key words: crocin young mice pneumonia MAPK/NF-κB pathway |
