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| 基于Box-Behnken响应面设计优选中药复方便乃通的提取工艺 |
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殷倩倩1,2, 邓秀平1,2, 钟雨虹1,2, 李鑫1,2, 李小英3, 刘锴3, 李嘉瑞3, 付茉莉3, 刘志东1,2, 李亦武3
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1.天津中医药大学组分中药国家重点实验室, 天津 301617;2.天津中医药大学现代中药发现与制剂技术教育部工程研究中心, 天津 301617;3.武汉同济现代医药科技股份有限公司, 武汉 430056
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| 摘要: |
| [目的] 利用Box-Behnken响应面设计法优化中药复方便乃通（BNT）的提取工艺。[方法] 基于单因素实验优选BNT提取工艺的影响因素及其范围，运用Box-Behnken响应面设计法构建三因素三水平实验设计方案，以番泻苷B、番泻苷A及橙皮苷的含量作为响应指标优选最佳提取工艺条件，并进行工艺验证。[结果] 中药复方BNT的最佳提取工艺条件为料液比1∶8，回流时间30 min，提取次数2次。[结论] 利用Box-Behnken响应面设计法优化中药复方BNT提取工艺方法稳定，可为BNT提取工艺提供参考。 |
| 关键词: 响应面设计 提取工艺 HPLC |
| DOI：10.11656/j.issn.1672-1519.2024.06.19 |
| 分类号:R284.2 |
| 基金项目: |
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| Optimization of the extraction process for Chinese herbal compound Biannaitong based on Box-Behnken response surface design |
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YIN Qianqian1,2, DENG Xiuping1,2, ZHONG Yuhong1,2, LI Xin1,2, LI Xiaoying3, LIU Kai3, LI Jiarui3, FU Moli3, LIU Zhidong1,2, LI Yiwu3
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1.State Key Laboratory of Component Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Modern Traditional Chinese Medicine Discovery and Formulation Technology Engineering Research Center of the Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;3.Wuhan Tongji Modern Pharmaceutical Technology Co., Ltd., Wuhan 430056, China
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| Abstract: |
| [Objective] To optimize the extraction process of Chinese medicine compound Biannaitong（BNT） by Box-Behnken response surface design. [Methods] Based on the single factor test，the influencing factors and range of BNT extraction process were optimized. The three-factor and three-level test design scheme was constructed by Box-Behnken response surface design method. The content of sennoside B，sennoside A and hesperidin was used as the response index to optimize the best extraction process conditions，and the process verification was carried out. [Results] The optimum extraction conditions of Chinese herbal compound BNT were as follows：solid-liquid ratio of 1∶8，reflux time of 30 min，extracting for 2 times. [Conclusion] The Box-Behnken response surface design method was used to optimize the BNT extraction process of traditional Chinese medicine compound. The method is stable and can provide a reference for the BNT extraction process. |
| Key words: response surface design extraction process HPLC |
