宣肺清络方对肺炎支原体肺炎痉挛性咳嗽大鼠肺组织病理学及炎性因子的影响

Effect of Xuanfei Qingluo Formula on lung histopathology and inflammatory factors in rats with MPP spasmodic cough

关键词:  肺炎支原体肺炎  宣肺清络方  炎症因子

Key words:  mycoplasma pneumoniae pneumonia  Xuanfei Qingluo Formula  inflammatory factor

摘要:

[目的] 探讨宣肺清络方对肺炎支原体肺炎（MPP）痉挛性咳嗽大鼠肺组织病理学及炎症因子的影响。[方法] 60只大鼠随机分为空白组、肺炎支原体肺炎组、模型组、阿奇霉素组、宣肺清络组、宣肺清络+阿奇霉素组，共计6组。采用肺炎支原体培养液滴鼻+烟熏法+辣椒素溶液雾化建立肺炎支原体肺炎感染后痉挛性咳嗽大鼠模型， 14 d造模成功后各组大鼠给予相应药物灌胃，连续治疗7 d后检测各组大鼠血清白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）水平及观察各组大鼠肺组织病理改变。[结果] 与正常组比较，肺炎支原体肺炎组、模型组血清中IL-6、IL-8、TNF-α含量表达增高，差异有统计学意义（P<0.05）；与模型组比较，各治疗组血清中IL-6、IL-8、TNF-α含量表达明显降低，差异有统计学意义（P<0.05）；在治疗干预后，各治疗组血清中IL-6、IL-8、TNF-α含量相互比较差异无统计学意义（P>0.05）。肺组织病理学显示，各治疗组组织病理改变较肺炎支原体肺炎组及模型组均有不同程度改善。[结论] 宣肺清络方可以减轻肺组织结构损害，减少间质内炎症细胞浸润，推测其机制可能与抑制血清炎性因子IL-6、IL-8、TNF-α含量有关。

Abstract:

[Objective] To investigate the mechanism of pulmonary histopathology and inflammatory factors in mycoplasma pneumoniae pneumonia（MPP） in rats with spasmodic cough. [Methods] Sixty rats were randomly divided into blank group，mycoplasma pneumoniae pneumonia group，model group，azithromycin group，Xuanfei Qingluo group，Xuanfei Qingluo + azithromycin group，a total of 6 groups. The rat model of spasmodic cough after mycoplasma pneumoniae pneumonia infection was established by nasal drops of mycoplasma pneumoniae culture + smoke method + capsaicin solution atomization，after 14 days of successful modeling，the rats in each group were given corresponding drugs by intragastric administration，after 7 days of continuous treatment，serum interleukin-6（IL-6），interleukin-8（IL-8），tumor necrosis factor-α（TNF-α） were detected in each group. The levels of TNF-α and the pathological changes of lung tissue in each group were observed. [Results] Compared with normal group，serum expressions of IL-6，IL-8 and TNF-α were increased in mycoplasma pneumoniae pneumonia group and model group，the difference was statistically significant（P<0.05）；compared with model group，the levels of IL-6，IL-8 and TNF-α in serum of all treatment groups were significantly decreased，and the difference was statistically significant（P<0.05）；after treatment intervention，there were no significant differences in serum contents of IL-6，IL-8 and TNF-α among treatment groups（P>0.05）. [Conclusion] Xuanfei Qingluo Formula can reduce the damage of lung tissue structure and the infiltration of inflammatory cells in the interstitial. It is speculated that the mechanism may be related to the inhibition of serum inflammatory factors IL-6，IL-8 and TNF-α.