黄精多糖对妊娠期高血压模型大鼠胎盘组织TGF-β/Smad信号通路的作用研究

Study on the effect of polygonatum polysaccharides on the TGF-β/Smad signal pathway in placental tissue of pregnancy induced hypertension rats

关键词:  妊娠期高血压  胎盘功能  黄精多糖  TGF-β/Smad信号通路

Key words:  pregnancy induced hypertension  placental function  polygonatum polysaccharide  TGF-β/Smad signal pathway

摘要:

[目的] 探讨黄精多糖对妊娠期高血压模型大鼠胎盘功能的影响与机制。[方法] 受孕雌性大鼠随机分为对照组、模型组、黄精多糖（低、中、高）剂量组（100、200、400 mg/kg）和硫酸镁组（100 mg/kg），每组10只。除对照组外，其余组大鼠皮下注射L-精氨酸甲酯构建妊娠期高血压模型。检测血压、24 h蛋白尿、胎鼠体质量、胎盘质量；苏木素-伊红（HE）检测胎盘组织病理学；酶联免疫吸附实验（ELISA）检测胎盘组织白介素（IL）-6、IL-10、肿瘤坏死因子-α（TNF-α）含量；Western Blot检测胎盘组织紧密连接蛋白-1（ZO-1）、封闭蛋白（OCLN）、血管内皮生长因子（VEGF）、转化生长因子-β1（TGF-β1）、p-Smad3、Smad3蛋白表达。[结果] 与对照组比较，模型组大鼠血压、24 h尿蛋白、IL-6、TNF-α含量及TGF-β1、p-Smad3蛋白表达显著升高，胎鼠体质量、胎盘质量、IL-10含量及ZO-1、OCLN、VEGF蛋白表达显著降低（P<0.05），且胎盘组织出现病理学损伤。与模型组比较，各给药组大鼠血压、24 h尿蛋白、IL-6、TNF-α含量及TGF-β1、p-Smad3蛋白表达显著降低，胎鼠体质量、胎盘质量、IL-10含量及ZO-1、OCLN、VEGF蛋白表达显著升高（P<0.05），同时胎盘组织病理学损伤减轻。[结论] 黄精多糖可抑制妊娠期高血压模型大鼠胎盘中TGF-β/Smad信号通路激活，改善胎盘功能障碍。

Abstract:

[Objective] To investigate the impacts and mechanism of polygonatum polysaccharide on placental function in pregnancy induced hypertension model rats. [Methods] Pregnant female rats were randomly grouped into control group，model group，polygonatum polysaccharide（low，medium and high） dose groups（100，200，400 mg/kg） and magnesium sulfate group（100 mg/kg），with 10 rats in each group. In addition to the control group，rats in other groups were subcutaneously injected with L-arginine methyl ester to build the hypertensive model of pregnancy. Blood pressure，24-hour proteinuria，fetal rat body mass and placenta weight were measured；hematoxylin-eosin（HE） was applied to detect the histopathology of placenta；the contents of interleukin（IL）-6，IL-10 and tumor necrosis factor-α（TNF-α） in placenta were detected by enzyme linked immunosorbent assay（ELISA）；Western Blot was applied to detect the expression of tight junction protein-1（ZO-1），blocking protein（OCLN），vascular endothelial growth factor（VEGF），transforming growth factor-β1（TGF-β1），p-Smad3 and Smad3 proteins in placenta. [Results] Compared with the control group，the blood pressure，24-hour urine protein，IL-6，TNF-α contents and TGF-β1，p-Smad3 protein expression in the model group were significantly higher；the body weight，placenta weight，IL-10 content and the expression of ZO-1，OCLN and VEGF protein of fetal rats were significantly lower（P<0.05），and pathological damage occurred in placenta tissue. Compared with the model group，the blood pressure，24-hour urine protein，IL-6，TNF-α contents and TGF-β1，p-Smad3 protein expression of rats in administration groups were significantly lower，the body weight，placenta weight，IL-10 content and the expression of ZO-1，OCLN and VEGF protein of fetal rats were significantly higher（P<0.05），and the pathological damage of placenta was alleviated. [Conclusion] Polygonatum polysaccharide can inhibit the activation of TGF-β/Smad signal pathway in the placenta of pregnancy induced hypertension model rats and improve the placental dysfunction.