HPLC-UV 法测定银杏内酯 A、B、C

HPLC-UV analysis of ginkgolide A,B,C

关键词:  银杏内酯  高效液相色谱  方法学验证

Key words:  ginkgolides  HPLC  methodology validation

摘要:

[目的] 建立高效液相色谱-紫外检测器（HPLC-UV）法测定银杏内酯A、B、C的浓度，并对其进行方法学验证。[方法] 考察了包括检测波长、8种不同的流动相体系及其比例、进样量、流速和柱温。方法验证涵盖了专属性、精密度、准确度、线性范围、重复性、系统适用性、加样回收率和稳定性。[结果] 色谱柱为Agilent TC-5 C18，检测波长为220 nm，流动相为水-甲醇（70∶30），进样量为20 μL，柱温30℃，流速为1.0 mL/min且线性关系良好，色谱峰之间无互相干扰。[结论] 该方法准确、有效，经济且易得，操作简单，为其他银杏内酯药物含量测定提供了可靠的参考。

Abstract:

[Objective] To establish and methodologically validate an HPLC-UV method for the determination of ginkgolides A，B，and C. The method was validated by the HPLC-UV method. [Methods] The investigation included detection wavelength，eight different mobile phase systems and their ratios，injection volume，flow rate and column temperature. The method validation examined the specificity， precision，accuracy，linear range，repeatability，system suitability，spiked recovery and stability. [Results] The separation was carried out on an Agilent TC-5 C₁₈ column at a detection wavelength of 220 nm with a mobile phase of water-methanol（70∶30） at an injection volume of 20 μL，a column temperature of 30 ℃，and a flow rate of 1.0 mL/min. The linearity of the method was good，and the peaks of the chromatograms did not interfere with each other. [Conclusion] The method is accurate，effective，economical，easy to obtain and simple to operate，and can provide a reference for the determination of other ginkgolides.