宣肺败毒颗粒增加T细胞数量减轻流感病毒肺炎的机制研究

The mechanism of Xuanfei Baidu Granules to treat influenza virus pneumonia by increasing T cells

关键词:  宣肺败毒颗粒  流感病毒(A/PR/8/34)  流感病毒肺炎  T细胞

Key words:  Xuanfei Baidu Granules  influenza virus(A/PR/8/34)  influenza virus pneumonia  T cell

摘要:

[目的] 确定宣肺败毒颗粒在低温高湿环境下，A/PR/8/34(H1N1)流感病毒肺炎小鼠模型的治疗效果及作用机制。[方法] 将ICR小鼠置于具有低温高湿环境的人工气候箱中，滴鼻给予流感病毒(A/PR/8/34)建立流感病毒肺炎模型。造模后第5天对小鼠每日给予宣肺败毒颗粒灌胃5 d，观察记录小鼠的行为学评分、体质量、脏器指数、肺部病毒载量、肺部影像学及形态学变化，蛋白印迹法检测炎症关键蛋白NF-κB的表达变化，流式细胞术检测宣肺败毒颗粒对小鼠免疫应答的调节。[结果] 与模型组相比，宣肺败毒颗粒给药后小鼠表现出活跃程度上升、大便性状正常、肺部病毒载量降低、小鼠肺部炎性渗出减少，p-NF-κB P65表达降低，CD4⁺和CD8⁺T细胞百分比增高。[结论] 宣肺败毒颗粒增加T细胞数量，减轻流感病毒肺炎小鼠的炎症反应。

Abstract:

[Objective] To establish a influenza virus(A/PR/8/34) pneumonia model under low temperature and high humidity,and evaluate the efficacy and mechanism of Xuanfei Baidu Granules(XFBD) against influenza. [Methods] ICR mice were placed in an artificial climate box with low temperature and high humidity,and influenza virus(A/PR/8/34) was given intranasally. On the 5th day after modeling,mice were given XFBD by gavage for 5 d. The behavioral score,body weight,organ index,pulmonary viral load,pulmonary imaging and morphological changes were observed and recorded. The expression of NF-κB,a key inflammatory protein, was detected by Western blot,and the regulation of XFBD on the immune response of mice was detected by flow cytometry. [Results] Compared with the model group,after administration of XFBD,mice showed increased activity,normal stool characteristics,decreased lung viral load,decreased inflammatory exudation in mice’s lungs,decreased expression of p-NF-κB P65,and increased percentages of CD4⁺ and CD8⁺T cells. [Conclusion] XFBD increased the amount of T cells,and inhibited the inflammation of the influenza virus pneumonia under cold and high humidity conditions.