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| 健脾化瘀方通过CD44/ERK信号通路抑制肝癌细胞上皮间质转化的作用机制 |
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鄞冰1,2,3, 冯坤良1,2,3, 王子豫1,2,3, 方崇锴1,2,3, 罗锐1,2,3, 赖永恒1,2,3, 钟崇1,2,3,4
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1.广州中医药大学第一临床医学院, 广州 510405;2.广州中医药大学岭南医学研究中心, 广州 510405;3.广州中医药大学第一附属医院胆胰外科, 广州 510405;4.中医证候全国重点实验室/广州中医药大学第一附属医院, 广州 510405
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| 摘要: |
| [目的] 观察健脾化瘀方对肝癌细胞上皮间质转化（EMT）及侵袭转移的影响，并探索其作用机制。[方法] 以高转移人肝癌细胞株MHCC97H为研究对象，设立对照组、10%含药血清组、20%含药血清组。含药血清干预24、48 h后，利用划痕实验及Transwell侵袭实验评估细胞迁移及侵袭能力，实时荧光定量聚合酶链式反应（Real-time PCR）及蛋白免疫印记法（Western Blot）检测EMT通路相关基因及蛋白的表达水平。[结果] 与对照组相比，含药血清组的MHCC97H细胞迁移下降，侵袭能力下降；健脾化瘀方含药血清可下调分化群44（CD44）、细胞外信号调节激酶（ERK）、锌指蛋白转录因子（Snail）、波形蛋白（Vimentin）、N-钙黏蛋白（N-cadherin）的基因表达，上调E-钙黏着蛋白（E-cadherin）基因表达。[结论] 健脾化瘀方含药血清可能通过抑制CD44/ERK通路的激活以抑制肝癌MHCC97H细胞EMT及迁移侵袭。 |
| 关键词: 肝癌细胞 健脾化瘀方 上皮间质转化 CD44/ERK |
| DOI：10.11656/j.issn.1672-1519.2026.06.16 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金项目（82274526）；广东省自然科学基金项目（2023A1515011069）；广州中医药大学“双一流”与高水平大学学科后备人才培育项目（A1-2601-22-415-023）。 |
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| Jianpi Huayu Decoction inhibits EMT in hepatoma cells via regulating the CD44/ERK pathway |
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YIN Bing1,2,3, FENG Kunliang1,2,3, WANG Ziyu1,2,3, FANG Chongkai1,2,3, LUO Rui1,2,3, LAI Yongheng1,2,3, ZHONG Chong1,2,3,4
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1.The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China;2.Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;3.Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China;4.State Key Laboratory of Traditional Chinese Medicine Syndrome/The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
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| Abstract: |
| [Objective] To study the inhibiting effect of Jianpi Huayu Decoction（JHD）in epithelial-mesenchymal transition（EMT）of hepatoma carcinoma cell，and to explore the possible mechanism behind it. [Methods] Culture MHCC97H cells in vitro，and divide the MHCC97H cells into three groups according to the stimulations（control group，10% of the drug-containing serum group，20% of the drug-containing serum group）.The cell migration and invasion abilities was evaluated by scratch test and Transwell invasion test， and Real-time PCR and Western Blot were used to detect the expression levels of genes and proteins related to the EMT pathway. [Results]Compared with control group，the migration and invasion ability of the drug-containing serum groups were significantly decreased. As well as，the mRNA and protein expression level of Cluster of differentiation 44（CD44），Extracellular regulated kinase （ERK），zinc finger protein transcription factor（Snail），Vimentin and N-cadherin were significantly decreased，while the expression of E-cadherin was increased. [Conclusion] JHD may inhibits EMT，migration，and invasion of hepatocellular carcinoma MHCC97H cells through inhibiting the activation of the CD44/ERK pathway. |
| Key words: hepatocellular carcinoma cell Jianpi Huayu Decoction EMT CD44/ERK |
