基于SIRT1/PGC-1α通路探讨针灸对筋膜疼痛综合征大鼠痛阈的影响

Exploring the effect of acupuncture on the pain threshold of rats with myofascial pain syndrome based on the SIRT1/PGC-1α pathway

关键词:  SIRT1/PGC-1α通路  针灸  筋膜疼痛综合征  痛阈

Key words:  SIRT1/PGC-1α pathway  acupuncture  myofascial pain syndrome  pain threshold

摘要:

目的 探究针灸治疗是否可调控SIRT1/PGC-1α通路影响筋膜疼痛综合征（MPS）大鼠痛阈。方法 将SD大鼠分为Con组、MPS组、非经非穴对照组、针灸组、针灸+EX527（Sirt1抑制剂）组和塞来昔布组，采用打击结合离心运动方式建立MPS模型。检测大鼠左下肢热缩足潜伏期和自发电活动。ELISA检测血清中肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6和IL-1β和脊髓组织前列腺素E2（PGE2）、P物质（SP）和5-羟色胺（5-HT）水平。HE染色检测肌肉组织病理形态学变化。免疫荧光法观察脊髓组织胶质纤维酸性蛋白（GFAP）和补体受体3（CR3）的抗体标志物（OX-42）的阳性表达情况。Western blot检测脊髓组织沉默信息调节因子1（SIRT1）和过氧化物酶体增殖物激活受体γ辅激活因子-1α（PGC-1α）蛋白表达水平。结果 与Con组比较，MPS组和非经非穴对照组大鼠肌肉组织有明显损伤，TWL值，脊髓组织SIRT1和PGC-1α蛋白表达水平降低，自发电位活动频率、血清TNF-α、IL-6和IL-1β水平、脊髓组织PGE2、SP和5-HT水平、GFAP和OX-42阳性细胞数量升高（P < 0.05）。与MPS组和非经非穴对照组比较，针灸组和塞来昔布组大鼠肌肉组织形态有明显改善，TWL值、脊髓组织SIRT1和PGC-1α蛋白表达水平升高，自发电位活动频率、血清TNF-α、IL-6和IL-1β水平、脊髓组织PGE2、SP和5-HT水平、GFAP和OX-42阳性细胞数量降低（P < 0.05）。EX527可削弱针灸治疗对MPS大鼠痛阈的改善作用（P < 0.05）；针灸组和塞来昔布组大鼠各项检测指标处于同一水平（P > 0.05）。结论 针灸治疗可能通过激活SIRT1/PGC-1α通路来降低MPS大鼠炎症，提高痛阈值，改善MPS。

Abstract:

Objective To explore whether acupuncture treatment can regulate the SIRT1/PGC-1α pathway and affect the pain threshold in rats with myofascial pain syndrome (MPS). Methods SD rats were separated into the Con group, the MPS group, the non-invasive and non-acupoint control group, the acupuncture group, the acupuncture +EX527 (Sirt1 inhibitor) group and the celecoxib group. The MPS model was established by combining striking with centrifugal motion. The thermal withdrawal latency and the spontaneous electricity activity of the left lower limb of rats were detected. ELISA was used to measure the levels of TNF-α, IL-6 and IL -1β in serum and PGE2, SP and 5-HT in spinal cord tissue. HE staining was implemented to measure the pathological morphological changes of muscle tissue. The positive expression of GFAP and OX-42 in spinal cord tissue was observed by immunofluorescence. The expression levels of SIRT1 and PGC-1α proteins in spinal cord tissue were detected by Western blot. Results Compared with the Con group and the non-invasive and non-acupoint control group, the muscle tissue of rats in the MPS group was clearly damaged, the TWL value, and the protein expression levels of SIRT1 and PGC-1α in the spinal cord tissue were lower, while the frequency of spontaneous potential activity, the levels of serum TNF-α, IL-6 and IL-1β, the levels of PGE2, SP and 5-HT in spinal cord tissue, and the numbers of GFAP and OX-42 positive cells were higher (P < 0.05). Compared with the MPS group and the non-invasive and non-acupoint control group, the muscle tissue morphology of rats in the acupuncture group and the celecoxib group was clearly improved, the TWL value, and the protein expression levels of SIRT1 and PGC-1α in the spinal cord tissue were higher, while the frequency of spontaneous potential activity, the levels of serum TNF-α, IL-6 and IL-1β, the levels of PGE2, SP and 5-HT in spinal cord tissue, and the numbers of GFAP and OX-42 positive cells were lower (P < 0.05). EX527 could weaken the improvement effect of acupuncture treatment on the pain threshold of MPS rats (P < 0.05). The various detection indicators of rats in the acupuncture group and the celecoxib group were at the same level (P > 0.05). Conclusion Acupuncture treatment may reduce inflammation in MPS rats, increase the pain threshold and improve MPS by activating the SIRT1/PGC-1α pathway.