基于PI3K/AKT信号通路探讨晚蚕沙外熨法对糖尿病胃轻瘫大鼠的影响

Exploring the effect of external ironing of silkworm sand on diabetic gastroparesis rats based on PI3K/AKT signaling pathway

关键词:  晚蚕沙外熨法  糖尿病胃轻瘫  细胞凋亡  PI3K/AKT信号通路

Key words:  silkworm external ironing  diabetic gastroparesis  apoptosis  PI3K/AKT signaling pathway

摘要:

[目的] 探讨晚蚕沙外熨法对糖尿病胃轻瘫（DGP）大鼠的作用机制。[方法] 随机选取8只大鼠作为空白组，其余大鼠构建DGP模型，造模成功后随机分为模型组、外熨组（晚蚕沙外熨法）和莫沙必利组（1.57 mg/kg）连续治疗2周。检测大鼠胃排空率、随机血糖水平，苏木精-伊红（HE）染色观察胃窦部形态变化，蛋白质免疫印迹法（Western blot）检测磷酸肌醇-3-激酶（PI3K）、磷酸化（p）-PI3K、蛋白激酶B（AKT）、p-AKT的蛋白表达，TUNEL染色检测大鼠胃窦部细胞凋亡。[结果] 与空白组比较，模型组胃排空率明显下降（P＜0.01），随机血糖水平升高（P＜0.01），胃窦部腺体结构紊乱，p-PI3K/PI3K、p-AKT/AKT、蛋白表达明显升高（P＜0.01），胃窦部细胞凋亡增加（P＜0.01）；与模型组比，外熨组胃排空率明显上升（P＜0.01），随机血糖下降（P＜0.05），胃窦部组织结构逐渐恢复，p-PI3K/PI3K、p-AKT/AKT蛋白表达明显降低（P＜0.01），胃窦部细胞凋亡减少（P＜0.01）。[结论] 晚蚕沙外熨法可降低DGP大鼠胃窦部细胞凋亡、改善胃动力以及降低随机血糖水平，其机制可能与调控PI3K/AKT信号通路有关。

Abstract:

[Objective] To explore the mechanism of the action of external ironing method on diabetic gastroparesis（DGP） rats. [Methods] The 8 rats were randomly selected as the blank group，and the rest of the rats were constructed as the DGP rat model，after successful modelling，they were randomly divided into the model group，the external ironing group（the external ironing method of the silkworm sand） and the mosapride group（1.57 mg/kg） and were treated continuously for 2 weeks. Detection of gastric emptying rate，random blood glucose level in rats，HE staining was used to observe the morphological changes in the gastric antrum，Western blot was used to detect the protein expression of phosphatidylinositol-3-kinase（PI3K），phosphorylated（p）-PI3K，protein kinase B（AKT），p-AKT，and TUNEL staining was used to detect the apoptosis of the gastric antrum in rats. [Results] Compared with the blank group，the gastric emptying rate of the model group decreased significantly（P＜0.01）；random blood glucose level increased（P＜0.01）；the glandular structure of the gastric sinus department was disturbed；p-PI3K/PI3K，p-AKT/AKT，protein expression increased significantly（P＜0.01）；apoptosis of the gastric sinus department was increased（P＜0.01）. Compared with the model group，gastric emptying rate of the externally ironed group increased significantly（P＜0.01）；random blood glucose decreased（P＜0.05）；the tissue structure of the gastric sinus department was gradually restored；the protein expression of p-PI3K/PI3K，p-AKT/AKT decreased significantly（P＜0.01）；the apoptosis of the gastric sinus department decreased（P＜0.01）. [Conclusion] External ironing of silkworm sand reduces apoptosis，improves gastric motility，and lowers random blood glucose levels in the gastric sinus of rats with diabetic gastroparesis，the mechanism of which may be related to the regulation of the PI3K/AKT signalling pathway.