| 摘要: |
| 目的: 建立决明子中降压降脂、保护肝脏的有效成分结合大黄酚的HPLC定量测定方法。方法: 选用SUPELCOSI-LTMLC18Column(5μm,4.6mm×250mm)色谱柱,流速1.0mL/min,测定波长254nm,柱温40℃。以大黄酚的含量为测定指标,流动相选甲醇-5%醋酸水溶液(75:25)。结果: 进样量在(0.08～3.20)μg范围内线性关系良好(γ=0.9997)。加样回收率:游离大黄酚为97.45%,RSD为2.21%;总大黄酚为102.14%,RSD为1.85%。结论: 本法专属性强,可用于决明子中大黄酚的含量测定。 |
| 关键词: 高效液相色谱法 决明子 大黄酚 |
| DOI：10.11656/j.issn.1673-9043.2002.03.38 |
| 分类号: |
| 基金项目:天津市科委重大科技攻关项目基金资助课题(0238037111) |
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| Content Analysis of Chrysophanol in Cassia Obtusifolia L.by HPLC |
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Qi Aidi,Yan Xuemei,Wang Hong
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Tianjin University of TCM, Tianjin 300193
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| Abstract: |
| Objective: To find a quantitative determination method by HPLC for examining the effective content of combining chrysophanol in Cassia obtusifolia L.,which could decrease blood pressure,blood lipid and protect the liver.Methods: SUPELCOSILTMLC18Column (5μm,4.6mm×250mm)was used with flow rate1.0 mL/min,detected wave 254 nm,chromatogratic column temperature 40℃ and mobile phase MeOH-5%AxOH(75:25).The content of chrysophanol was detected. Results: The linear relationship was fine (γ=0.9997) in (0.08～3.20)μg. The recovery rate of free chrysophanol was 97.45%,RSD2.21%;of total chrysophanol was102.14%,RSD1.85%.Conclusion: This method is specific and can be used to detect the content of chrysophanol. |
| Key words: HPLC chrysophanol Cassia obtusifolia |
