糖尿病兔胆囊细胞超微结构改变

The cholecystic cell ultrastructural change of the Diabetic Rabbits

关键词:  兔糖尿病模型  四氧嘧啶  胆囊微血管病变

Key words:  The rabbits diabetes models  alloxan  the cholecystic microvascular pathological changer

摘要:

[目的]观察四氧嘧啶致糖尿病兔的胆囊细胞超微结构的改变。[方法]选择大耳白兔26只,随机分为2组,即正常组、四氧嘧啶致糖尿病组,根据血糖情况,适量皮下注射胰岛素,控制餐后血糖在20mmol/L左右,饲养6周后处死观察胆囊超微结构的变化。[结果]糖尿病组14只入组,终末存活11只,电镜下发现糖尿病兔发生胆囊微血管内皮细胞功能不良,内皮细胞核呈坏死病理改变,胞浆有部分崩解,内皮细胞基底板呈高度增厚。胆囊平滑肌细胞可见细胞凋亡,细胞间可见成堆的大量髓磷体。胆囊上皮细胞、胆囊壁微血管内皮细胞均可见到超微结构的改变。[结论]实验结果证实糖尿病兔早期胆囊微血管内皮细胞及胆囊平滑肌细胞均发生超微结构的改变。

Abstract:

[Objective] To observe the cholecystic cell ultrastructural change of the diabetic rabbits induced by Alloxan. [Methods] Twenty-six rabbits were divided randomly into 2 groups:normal group (N group) and the diabetes mellitus group induced by Alloxan (DM group). In DM group,we have controlled the rabbits' blood sugar in 20 mmol/L.We observed the cholecystic cell ltrastructural change of the diabetic rabbits in six weeks. [Result] There were 11 rabbits survived in DM group. We have found cholecystic cell ultrastructural change of the diabetic rabbits in the electron microscope. There were malfunction about cholecystic microvessel, nucleus necrosised,cell plasm partly collapsed,high increased thickness in endothelial cell basal lamina. The cholecystic smooth muscle cell apoptosis and mass marrow phosphorus body between the cell [Conclusion] Rabbits in early DM had the cell ultra structural change in cholecystic microvascular endothelial cell and smooth muscle cell.