荷叶总黄酮苷质量控制研究*

Investigation on Quality Control of Total Glucoside of Flavone in Folium Nelumbinis

关键词:  荷叶黄酮苷  高效液相色谱法  水解

Key words:  total flavone glucoside of Folium Nellumbinis  HPLC  hydrolysis

摘要:

[目的]建立荷叶提取物中黄酮类化合物的高效液相色谱测定方法。[方法]药材经提取水解后用HPLC通过测定其槲皮素的含量来确定荷叶提取物中总黄酮的含量,采用Shim-packCLC-ODS(4.6mm×150mm,5μm)色谱柱,甲醇-水-磷酸(50:50:0.1)为流动相,流速0.8mL/min,检测波长360nm。[结果]槲皮素在0.013～0.130μg范围内呈良好线性关系(r=0.9999),荷叶提取物中槲皮素的平均含量为4.94%,RSD=1.67% ;平均回收率为99.15%,RSD=0.60% 。[结论]此测定方法简便快捷、精密度高、准确性好,可以作为荷叶中总黄酮的含量测定方法,以控制荷叶黄酮苷的质量。

Abstract:

[Objective] To establish the determination method of flavonoids compound in the extract of Folium Nelumbinis with HPLC.[Methods] The total quereetin content in the Folium Nelumbinis was determined with HPLC after hydrolysis.The procedure was as follows:the chromatographic column was Shim-pack CLC-ODS (4.6 mm×150 mm,5μm)g mobile phase was methanol-water-phosphoric acid (50:50:0.1)g flow rate was 0.8 mL/ming detection wavelength was 360 nm.[Result]The content of sophoretin showed a good linear relationship at the range of 0.013～0.13μg (R=0.999 9).In the extract of Folium Nelumbinis the average content of sophoretin was 4.94%,RSD=1.67%.The average recovery rate was 99.15%,RSD=0.60%.[Conclusion] This technique is a convenient and simple method with high accuracy.The method can be used for the determination of the total flavonoids in the extract of Folium Nelumbinis and for the controlling the quality of the total glycoside of it.