血必净注射液对脓毒症大鼠器官功能及死亡率的影响*

Effects of xuebijing injection on organ function and mortality in rats with sepsis

关键词:  血必净注射液  脓毒症  盲肠结扎穿孔  器官功能  死亡率

Key words:  Xuebijing injection  sepsisf cecum ligation and puncture  organ functionf mortality

摘要:

[目的]观察血必净注射液对脓毒症大鼠多器官功能及死亡率的影响。[方法]采用大鼠盲肠结扎穿孔(CLP)模型。雄性Wistar大鼠96只按随机数字表法分为正常对照组(n=8)、假手术组(n=8)、模型对照组(n=40),血必净治疗组(n=40),后两组按观察时间点分为2、8、24、48和72h亚组。另设实验观察血必净注射液对脓毒症大鼠的治疗效果,观察CLP术后7d存活率。[结果]血必净治疗组血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、尿素氮(BUN)、肌酐(Cr)、肌酸激酶(CK)及心肌型肌酸激酶同功酶(CK-MB)水平呈不同程度降低;与模型未治疗组比较,血必净治疗组及联合治疗组动物死亡率显著降低。[结论]血必净注射液能够明显降低脓毒症大鼠的死亡率,并对重要器官功能具有保护作用。

Abstract:

[Objective] To investigate the effects of Xuebijing (cleaning the blood) injection on them ultiple organ function and mortality in ratswith sepsis.[Methods] Sepsis was reproduced in ratswith cecum ligation and puncture (CLP).Ninety-six male W istarratswererandom lydivided intonorm alcontrolgroup (n=8),sham-operation group (n=8),CLP model group (n=40),Xuebijing-treated group (n=40).The two latter groups were further divided into five groups of 2,8,24,48,and 72-hour subgroups with 8 ratsin each.In addition,another experiments were performed to observe the effect of treatment of Xuebijing on the survivalrate.[Results] Serum levels of ALT,AST,blood urea nitrogen (BUN),creatinine (Cr),creatine kinase (CK) and MB isoenzyme ofcreatine kinase (CK-MB) were significantly increased with certain extentat different intervals.Early treatment with Xuebijing could markedly improved serum biochemical param eterspartly.Treatm entwith Xuebijing or/and antibiotic could significantly improve the survivalrates in septic rats.[Conclusion] These finding sindicates that Xuebijing injection can obviously decline the mortality of septicrats,and hasplayed an important role in protecting against the multiple organ dysfunction.