中药(心身1号)对应激性溃疡模型大鼠保护机制的实验研究*

Experimental study on the protective mechanism of traditional Chinese medicine (Xinshen Ⅰ Prescription) against stress ulceration in rats

关键词:  心身1号  应激性溃疡  P物质

Key words:  stress ulceration  Xinshen Ⅰ Prescription  experimental study

摘要:

[目的]研究中药心身1号对应激性溃疡病证结合大鼠的干预效应并探讨其作用机制。[方法]采用Wistar大鼠造模,分为模型组、对照组、中药组3组。测量造模前后胃液pH值、胃黏膜溃疡指数、脑肠肽P物质。[结果]pH值测定模型组升高,中药组与模型组比较差别不显著;溃疡指数中药组较模型组明显为低;血浆、脑组织、胃组织中P物质含量中药组较模型组明显为低。[结论]心身1号的作用机制可能与调节血浆、脑组织及胃黏膜中P物质的过度升高因素有关。

Abstract:

[Objection] To explore the protective mechanism of Xinshen (spirit and body) Ⅰ Prescription against stress ulceration in rats.[Methods] All the rats were randomly divided into three groups:model group,normal group,Xinshen Ⅰ group.The rats of Xinshen Ⅰ Prescription group were administrated with Xinshen Ⅰ Prescription and that of model and normal group with normal saline.The general state,the gastric acid and mucosa change and substance P change of cerebral enteropetide and curative effect of Xinshen Ⅰ Prescription were observed.[Results] The pH value of model group was obviously higher than that of normal group (P<0.05) and the pH value of model group was not obviously different compared with that of Xinshen Ⅰ Prescription group (P>0.05).So Xinshen Ⅰ Prescription didn't enhance the pH value of gastric juice. The mucosa ulcer index of model group was obviously higher than that of normal group (P<0.01) and the mucosa ulcer index of Xinshen Ⅰ Prescription group was obviously lower than that of model group (P<0.05).Xinshen Ⅰ Prescription might obviously decrease the content of substance P in plasma,brain tissue and stomach mucosa (P<0.01).[Conclusion] Xinshen Ⅰ Prescription may obviously protect the stomach mucous membrane,the integrality of cellular construction, and prevent the formation of ulcer.Its mechanism may be related to the excessive high content of substance P in the blood plasma,brain tissue and stomach mucosa.