| 摘要: |
| [目的]建立活络镇痛片质量标准。[方法]采用薄层色谱(TLC)法对处方中当归、白芷、防风进行鉴别,用高效液相色谱(HPLC)法测定白芷中欧前胡素的含量。资生堂MGC18柱(150mm×4.6mm,5μm)色谱柱;检测波长248nm;流速1mL/min;以甲醇-水(63:37)为流动相。[结果]在TLC色谱中,均能检出当归、白芷、防风;欧前胡素在0.224～5.6μg范围内线性关系良好(r=0.9999),平均回收率为100.7%。[结论]该方法简单、准确、专属性强,可作为活络镇痛片质量控制方法。 |
| 关键词: 活络镇痛片 薄层鉴别 高效液相色谱法 |
| DOI：10.11656/j.issn.1673-9043.2008.01.12 |
| 分类号: |
| 基金项目: |
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| Study on the quality standard of Huoluo Zhentong tablets |
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GAO Mo,GAO Wen-Yuan,YOU Qiang-Zhen
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College of Pharmaceuticals & Biotechnology Tianjin University, Tianjin 300072, China
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| Abstract: |
| [Objective] To establish the quality standard of Huoluo Zhentong tablets which was composed of Radix Angelicae Sinensis,Radix Angelicae Dahuricae and Radix Saposhnikoviae etc.[Methods] Using TLC to identify Radix Angelicae Sinensis,Radix Angelicae Dahuricae and Radix Saposhnikoviaed Using HPLC to quantitate the imperatorin in Radix Angelicae Dahuricae.[Result] Radix Angelicae Sinensis,Radix Angelicae Dahuricae and Radix Saposhnikoviae could be easily examined by TLC.Imperatorin had a good linear relationship in the range of 0.224～5.6μg (r=0.9999).The average recovery rate was 100.7%.[Conclusion] The method is accurate and specific.It can be used for qualitative of Huoluo Zhentong tablets. |
| Key words: Huoluo Zhentong tablets TLC HPLC |
