糖尿病大鼠难治性溃疡模型的复制及病理学观察*

Effect of shengji corium elephatis mastic on the terminal glycosylation production and acceptor of granulation tissue of ulcus raw surface in rats with diabetes

关键词:  糖尿病  难治性溃疡  动物模型

Key words:  Shengji corium elephatis mastic  diabetes ulcus  terminal glycosylation production  RAGE

摘要:

[目的]建立糖尿病大鼠难治性溃疡动物模型。[方法]在糖尿病大鼠背部造成圆形无菌创面(直径2cm),分别于1、3、7、14、21d监测血糖同时进行创面照相,计算机图象分析系统分析溃疡的大小及深度,创面愈合时间。[结果]糖尿病组动物的愈合速度明显低于空白对照组动物,通过对两组肉芽组织的电镜观察糖尿病组动物的成纤维细胞出现的时间和数量明显晚于空白对照组。[结论]以1%键脲菌素能成功建立糖尿病大鼠难治性溃疡动物模型。

Abstract:

[Objection] To dynamic observe the terminal glycosylation production and acceptor of granulation tissue of ulcus raw surface in rats with diabetes and explore the effect mechanism of [Methods] To establish the animal model of diabetes ulcer. The model rats were demixing ran-domly divided into diabetes control group, control group, Vasogen group, Shengji corium elephatis and obtain fresh granulation tissue by using operation method. To determine the terminal glyco-sylation production content by using RT-PCR and immunohistochemical method. [Results] The content of terminal glycosylation production wasn't obviously differentiation on the rats of every of mean tendency, the content of RAGE in Shengji corium elephatis mastic group was obviously may inhibit the production of RAGE with the prolongation of time. The expression of RAGE was low in rats of every diabetes group in twenty day. [Conclusion] Shengji corium elephatis mastic prolongation of time.