| 摘要: |
| [目的] 建立大鼠灌胃总姜黄素溶液后血浆中姜黄素的高效液相色谱(HPLC)测定方法,进行大鼠体内药动学研究。[方法] 大鼠灌胃总姜黄素溶液后,不同时间眼底静脉丛取血,制备血浆,血浆样品经液—液萃取以后,以大黄素为内标,进行HPLC检测。色谱条件Hypersil C18柱(250mm×4.6mm,5μm),流动相为乙腈—2.0%醋酸水溶液(46:54),检测波长为UV420nm。[结果] 姜黄素的线性范围为2~300mg/L,最低定量限为2mg/L,日内、日间峰面积(RSD)分别≤5.08%和≤7.93%。[结论] 此法灵敏、快速、准确,可用于大鼠血浆中姜黄素浓度的测定和临床前药代动力学研究。 |
| 关键词: 姜黄素 液相色谱法 液—液萃取 |
| DOI：10.11656/j.issn.1673-9043.2008.04.13 |
| 分类号: |
| 基金项目: |
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| In vivo pharmaco kinetics study of total curcumine in rats |
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ZHU Qing-hua, LIU Cai-xia, CHEN Jing
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Tianjin University of TCM, Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin 300193, China
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| Abstract: |
| [Objective] To carry out a in vivo pharmacokinetics study on total curcumine and establish a HPLC determination method of blood curcumine.[Methods] The plasma of total curcumine was prepared and the content of total curcumine determined.Using Hypersil C18 column (250 mm×4.6 mm,5 um),the mobile phase was a mixture of acetonitrile-acetic acid (46:54) and the wavelength was 420 nm.[Results] The line range of total curcumine was 2~300 ug/L,the lowest limit of quantity was 2 ug/L and RSD was between 5.08% and 7.93%. [Conclusion] The method is simple,accurate and can be used to control the quality of total curcumine and in pre-clinic pharmacokinetics study. |
| Key words: total curcumine chromatography liquid-liquid extraction |
