白介素1β在大鼠卵泡发育中表达及功血宁Ⅱ号冲剂的影响*

Expression of interleukin 1β during development of follicle and the effect of GongXueNing Chongji No. Ⅱ on it

关键词:  白介素1β  功血宁Ⅱ号冲剂  卵泡发育

Key words:  interleukin 1β  GongXue Ning Chongji No.Ⅱ  development of follicle

摘要:

[目的]观察白介素1β(IL-1β)在大鼠卵泡发育过程中的表达以及功血宁Ⅱ号冲剂对其表达的影响,探讨该药促进卵泡发育诱发排卵的作用机制。[方法]将未成年雌性大鼠随机分为模型对照组、阳性对照组和功血宁Ⅱ号高、低剂量组,分别给予蒸馏水、安坤赞育丸和功血宁Ⅱ号冲剂,14d后皮下注射妊马血清促性腺激素(PMSG)+人绒毛膜促性腺激素(hCG)制备诱发排卵大鼠的模型。分别在卵泡发育期和排卵前期处死动物摘取卵巢,常规制备卵巢切片,免疫组化法检测各组动物卵泡不同发育时期IL-1β表达。[结果]IL-1β在模型对照组中的表达,排卵前期的阳性细胞面积、阳性面积率、积分光密度、平均光密度均显著高于卵泡发育期(P<0.05);功血宁Ⅱ号组在卵泡发育期和排卵前期IL-1β表达的上述指标均明显高于模型对照组(P<0.05或P<0.01),排卵前期的上述指标均明显高于相应卵泡发育期(P<0.05或P<0.01),其中高剂量组的作用较低剂量组和阳性对照组显著。[结论]IL-1β在大鼠卵泡发育过程中均有表达并逐渐增强。功血宁Ⅱ号冲剂能够显著提高IL-1β的表达,通过其参与卵母细胞成熟,诱导卵巢排卵因子的产生,在卵泡破裂激发排卵过程中发挥作用,促进卵泡发育诱发排卵。

Abstract:

[Objective] To observe the expression of interleukin 1β (IL-1β) during the development of follicle and the effect of GonXueNing Chonji No.Ⅱ (Stopping the hemorrhage from the uterus) on it.[Methods] immature female rats were randomly divided into model control group,positive control group,GongXueNing Ⅱ high and low dosage group.They were treated with distilled water,Ankunzanyu Pill and GongXueNing Chongji No.Ⅱ (taken medicine after infused in boiling water) respectively.14 days later a model of ovulation in rats was induced by subcutaneous FMSG+hCG.The animals were sacrificed at development period of follicle and preovulatory period and their ovaries were harvested.The expression of IL-1β pof pathologic section in the different development periods was measured with immunohistochemistry.[Results] In the model group the IL-1β pexpression,the positive cell area,the rate of positive area,the integrated optical density,the average optical density in preovulatory period were obviously higher than that in the development period of follicles (P<0.05).In GongXueNing No.II group the above parameters of IL-1β p expression at the development period of follicles and preovulatory period were all higher than the model control group (P<0.05 or P<0.01).All the parameters described above at preovulatory period were obviously higher than that at the corresponding period of follicle development (P<0.05 or P<0.01).Among them,the effect of high dosage group was more obvious than that in low dosage group and the positive control group.[Conclusion] IL-1β pwas expressed and increased gradually during the process of follicle development.GongXue Ning Chongji No.B can significantly increase the expression of IL-1β pand can accelerate the development of follicle and induce the ovulation.