丹酚酸B对离体内皮祖细胞黏附、趋化和增殖的影响*

Effects of salvianolic acid B on conglutination,migration and proliferation of endothelial progenitor Cells (EPCs) in vitro

关键词:  丹酚酸B  内皮祖细胞  黏附  趋化  增殖

Key words:  Key words salvianolic acid B (SaIB)  endothelial  progenitor cels (EPCs)  conglutination  migration  proliferation

摘要:

[目的]探讨中药单体丹酚酸B对离体内皮祖细胞(EPCs)黏附、趋化和增殖的影响。[方法]密度梯度离心法和贴壁筛选法培养、纯化EPCs,免疫细胞化学法(CD₃₄/CD₁₃₃/)鉴定。丹酚酸B最佳药物浓度干预的EPCs,DiI染色法,Transewell小室与DAPI染色法和Brdu免疫细胞化学法测定EPCs的黏附、趋化和增殖能力。[结果]丹酚酸B组EPCs的黏附细胞数量为(37.00±3.27),24h和48h趋化的细胞数量分别为(26.32±2.66)和(53.41±3.85),增殖的细胞数量为(41.17±3.43),与对照组比较差异均具有统计学意义(P<0.05)。[结论]丹酚酸B能够有效促进离体内皮祖细胞的黏附、趋化和增殖能力。

Abstract:

[Objective] To invistigate the effects of Salvianolic acid B on conglutination,migration and proliferation of Endothelial.Progenitor Cells (EPCs) In Vitro.[Methods] Cultivation and purification of EPCs in vitro was by density-gradient centrifugation and plastic adherence method,and identified by immunofluorescence and immunocytochemistry (CD₃₄/CD,₁₃₃).The conglutination,migration and proliferation of EPCs were examined by DiI stain,Transewell and DAPI stain and Brdu immunofluorescence and immunocytochemistry method respectively.[Results] The Cell count of conglutination was (37.00±3.27),those of migration at 24 horus and 48 hours were (26.32±2.66) and (53.41±3.85) respectively and that of proliferation was (41.17±3.43),which increased remarkably comparing with the control group.[Conclusion] Salvianolic acid B could improve markedly the conglutination,migration and proliferation ability of Endothelial Progenitor Cells (EPCs) In Vitro.