| 摘要: |
| [目的]建立以高效液相色谱法测定小儿消退糖浆中的大黄酚含量的方法。[方法]色谱为C18色谱柱(4 mm×250 mm,5μm),流动相为甲醇—水—磷酸—戊烷磺酸钠(80:20:0.1:0.1),流速1.0 mL/min,检测波长为254 nm,柱温为室温。[结果]大黄酚检测浓度在5～25 mg/L范围内与峰面积值线性关系良好(r=0.999 9),平均回收率为100.13%(RSD=1.1%)。[结论]本方法可用于消食退热糖浆的质量检测。 |
| 关键词: 高效液相色谱法 小儿消退糖浆 大黄酚 含量 |
| DOI：10.11656/j.issn.1673-9043.2009.02.13 |
| 分类号: |
| 基金项目: |
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| Determination of Chrysophanol in Pedo-digestion & fever- bringing-down Syrup by HPLC |
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WANG Shu-bei,YUAN Heng-jie,QI Ai-di
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Department of Pharmacy, GeneraL HospitaL of Tianjin Medical University, Tianjin 300052, China
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| Abstract: |
| [Objective] To establish HPLC method for determination of chrysophanol in pedo-digestion &fever-bringing-down syrup.[Method] The determination was performed on C18 column,the mobile phase consisted of methanol-water-phosphoric acid-sodimn pentanesulfonic acid (80:20:0.1:0.1 g) with flow rate at 1.0 mL/min and detection wavelength at 254 nm under room tempterature.[Results] Chrysophanol had good linear relation in the range of 5～25 mg/L (r=0.999 9),the average recovery was 100.13% (RSD=1.1%).[Conclusion] The method can be used to control the quality of digestion& bringing down a fever syrup. |
| Key words: HPLC Digestion&bringing down a fever syrup Chrysophanol determination |
