丹酚酸B干预大鼠急性心肌梗死后心肌细胞失巢凋亡的研究*

Study on effect of Salvianolic acid B on apoptosis of myocardial cells after acute myocardial infarction in rats

关键词:  丹酚酸B  失巢凋亡  血浆层黏液连蛋白  Ⅲ型前胶原氨基端肽  整合素  黏着斑激酶

Key words:  Salvianolic acid B  apoptosis  LN  PⅢNP  Integrin  FAK

摘要:

[目的]研究丹酚酸B(SalB)对急性心肌梗死大鼠心肌细胞失巢凋亡的作用及其分子机制。[方法]结扎冠脉左前降支复制大鼠急性心肌梗死(AMI)模型,实验分为假手术组、模型组和丹酚酸B组,其中丹酚酸B组于术后灌胃120mg/(kg·d),连续给药4周。各组分别于术后1、2、4周,采用放射免疫法检测血浆层黏连蛋白(LN)和Ⅲ型前胶原氨基端肽(PⅢNP)的含量;采用Masson染色法检测非梗死区胶原容积分数(CVF);免疫组织化学法检测心肌组织整合素(FAK)和黏着斑激酶的表达;用原位末端标记(TUNEL)法检测心肌细胞的凋亡。[结果]与假手术组相比较,血浆LN、PⅢNP水平均升高(P<0.01),非梗死区CVF升高,整合素和FAK的表达降低,心肌细胞凋亡指数增加(P<0.05)。与模型组相比较,血浆LN、PⅢNP水平均有所降低(P<0.01),非梗死区CVF降低,整合素和FAK的表达增加,心肌细胞凋亡指数降低(P<0.05)。[结论]丹酚酸B可以减少急性心肌梗死大鼠心肌细胞的失巢凋亡。

Abstract:

[Objective] To study the effect and mechanism of Salvianolic acid B (SalB) on the apoptosis of myocardial cells after acute myocardial infarction (AMI) in Rats.[Methods] Myocardial infarction was induced by ligating the descending left coronary artery in rats.The rats were divided into sham-operated group,model group and Sal B group.Sal B was given with 120 mg/ (kg·d) by gastric perfusion for 4 weeks after infarction in Sal B group.The animals in each group were killed after 1 week,2 weeks and 4 weeks of operation respectively.Radioimmunoassay was used to measure the content of laminin (LN) and ProcollagenⅢN-terminal Peptide (PⅢNP) in serum of rats;Masson staining was used to determine collagen volume fraction (CVF) in non-infarcted regions;Immunohistochemical method was applied to examine the expression of integrin and focal adhesion kinase (FAK) in myocardial tissues;TUNEL was used to detect the changes of apoptosis of myocardial cells.[Results] Compared with sham-operated group,content of LN and PⅢNP in serum increased (P<0.01) 1 week,2 weeks and 4 weeks after operation in model group,CVF increased,expression of integrin and FAK decreased,myocardial apoptosis index increased (P<0.05) ;compared with model group,content of LN and PⅢNP in serum was reduced (P<0.01) 1 week,2 weeks and 4 weeks after operation in Sal B group,CVF was reduced,expression of integrin and FAK was increased,apoptosis of myocardial cells index was reduced (P<0.05).[Conclusion] Sal B can reduce the apoptosis of myocardial cells after AMI in rats,and its mechanism may be related to reducing the content of LN and PⅢNP in serum,reducing changes of collagen and extracellular matrix in myocardial tissue,and activating integrin and FAK signaling pathway.