银杏内酯B对动脉粥样硬化家兔模型血清CRP和主动脉VCAM-1 mRNA的影响*

Effects of ginkgolide B on serum CRP and aortic VCAM-1 mRNA in rabbit with atherosclerosis

关键词:  动脉粥样硬化  银杏内酯B  C反应蛋白  血管细胞黏附分子-1

Key words:  Atherosclerosis  Ginkgolide B  CRP  VCAM-1

摘要:

[目的]探讨银杏内酯B(EGB)对动脉粥样硬化家兔模型血清反应蛋白(CRP)含量和主动脉血管细胞黏附分子-1(VCAM-1 mRNA)表达的影响。[方法]通过高脂饲料喂养8周复制动脉粥样硬化家兔模型,将35只家兔随机分为对照组、模型组和银杏内酯B组,8周后,银杏内酯B组加喂EGB5mg(/kg·d),继续喂养8周。处死前耳缘静脉取血,采用ELISA法检测血清CRP含量;处死后取主动脉,采用实时定量PCR法检测VCAM-1 mR-NA表达。[结果]模型组血清CRP含量比对照组升高,银杏内酯B组血清CRP含量降低,与模型组相比两组差异均有显著性,P<0.01。模型组VCAM-1 mRNA表达比对照组升高,银杏内酯B组VCAM-1 mRNA表达降低,与模型组相比两组差异均有显著性,P<0.01。[结论]银杏内酯B能够降低动脉粥样硬化家兔模型血清CRP含量,有效抑制动脉粥样硬化家兔模型主动脉VCAM-1 mRNA表达,从而起到防治动脉粥样硬化的目的。

Abstract:

[Objective] To explore the effect of ginkgolides B on the content of serum CRP and the expression of aortic VCAM 1-gene in rabbits with atherosclerosis.[Methods] High fat feed was use to induce rabbit model of atherosclerosis.Thirty-five rabbits were divided into control (ordinary),model group,and ginkgolides B group,the last two groups were given high fat feed for eight weeks.After that the rabbits in ginkgolides B group were fed with EGB,5 mg/ (kg·d).They were continued to feed for another eight weeks.Blood sample was extracted from ear margin vein before death.Enzyme-linked immunosorbent assay (ELISA) was used to detect the rabbit serum CRP levels.After death,a sample of aortic artery was taken to detect VACM-1 gene expression by PCR method.[Results] The CRP levels of model group was obviously higher than that of control group;serum CRP content of ginkgolides B group was lower than that of model group.There was a significant difference between both groups (P<0.01).VCAM-1 gene expression in model group was higher than control,ginkgolides B group was lower than model group,obviously difference was found between two groups (P<0.01).[Conclusion] Ginkgolides B can reduce serum CRP levels in rabbits with atherosclerosis,inhibit the aortic VCAM-1 gene expression effectively,thus Preventing the development of arteriosclerosis.