红缘拟层孔菌最佳母种培养基筛选及液体发酵培养液配方的初步研究

Screening of mother medium and the optimal media composition for the liquid fermentation of fomitopsis pinicola

关键词:  红缘拟层孔菌  多糖  母种培养基  液体发酵  正交设计实验

Key words:  Fomitopsis pinicola  polysaccharide  mother medium  liquid fermentation  orthogonal test

摘要:

[目的]筛选并优化红缘拟层孔菌的最佳母种培养基和液体发酵培养基的配方。[方法]将等量红缘拟层孔菌分别接种4种不同的固体培养基上,定期观察各组培养基上菌丝的长势和老化度。以胞内多糖和菌丝体生物量为指标,利用单因素和正交设计实验对红缘拟层孔菌的液体培养基配方进行筛选及优化。[结果]红缘拟层孔菌液体发酵培养基最佳配方为(10g/L):大米粉5,酵母粉1,磷酸二氢钾0.1,硫酸镁0.15,维生素B10.04。[结论]优选出了红缘拟层孔菌的最佳母种培养基和液体发酵的培养基,为下一步液体培养条件的探索奠定了基础。

Abstract:

[Objective] To select the mother medium composition and the optimal media composition for the liquid fermentation of Fomitopsis pinicola.[Methods] The same amount of Fomitopsis pinicola was cultured to compare their growth rate and aging degree by four different solid media.Taking the mycelial biomass and production of polysaccharide as the detecting indices,the optimum submerged fermentation parameters for Phellinus igniarius were studied by single factor test and orthogonal test.[Results] Fomitopsis pinicola growing on chemical media grew quickly,so the chemical media was suitable to be used as expanding species for reproduction.The aging degree of hypha growing on chemical media was most slowly,so bran media was suitable to be used as conservation.The optimum formula for liquid fermentation (g/100mL) were:rise powder 5,yeast 1,KH₂POSUB>3 0.1,MgSOSUB>4 0.15,VB1 0.040.[Conclusion] Our study has selected the mother medium composition and the optimal media composition for the liquid fermentation of Fomitopsis pinicola and provided the scientific basis for the further research of Fomitopsis pinicola.