中药单体对HepG2细胞株脂代谢相关基因mRNA表达的影响*

Effect of the monomer of traditional Chinese medicine on the lipid-related genes expression in hepatic cell line of HepG2

关键词:  丹酚酸B  姜黄素  荷叶碱  何首乌碱  脂代谢相关基因  逆转录-聚合酶链反应

Key words:  salvianolic acid B  curcumine  nuciferine  gold theragan base  lipid-related genes  RT-PCR

摘要:

[目的]探讨疏肝导浊方中,中药单体丹酚酸B、姜黄素、荷叶碱和何首乌碱对HepG2肝细胞株脂代谢相关基因(PPARα、HL、ApoB100、ApoAII及ApoCII)表达的影响。[方法]以HepG2细胞株为研究对象,分为空白对照组和中药单体丹酚酸B、姜黄素、荷叶碱和何首乌碱5组,采用逆转录-聚合酶链反应(RT-PCR)方法检测基因PPARα及载脂蛋白B100、AII及CII的相对表达情况,并用β-actin作为内参照予以矫正。[结果]丹酚酸B组、姜黄素组以及荷叶碱组能够刺激HepG2细胞PPARα mRNA表达水平升高,而何首乌碱降低了PPARα mRNA表达;只有丹酚酸B能够刺激HepG2细胞HLmRNA水平升高;丹酚酸B、姜黄素、何首乌碱、荷叶碱均能刺激HepG2细胞株ApoB100 mRNA表达上调;姜黄素和何首乌碱能够刺激ApoAIImRNA表达上调;丹酚酸B和何首乌碱可升高ApoCII mRNA表达水平。[结论]疏肝导浊方药治疗脂肪肝的机制可能是增加脂代谢相关基因的表达水平,但每个单味药所调节基因的表达(上调和下调)各不相同。

Abstract:

[Objective] To study the effect of monomer of traditional Chinese medicine on the lipid-related genes expression (PPARα, HL, ApoB100, ApoAII and ApoCII) on the hepatic cell line of HepG2. [Methods] HepG2 cells in vitro were chosen as the study object. They were divided into 5 groups:salvianolic acid B group, curcumine group, nuciferine group, gold theragan base group and normal group. The abundance of PPARα, HL, ApoB100, ApoAⅡ and ApoCⅡ mRNA in hepatic cell line of HepG2 were measured by RT- PCR with β-actin as the internal standard. [Results] Salvianolic acid B, curcumine and nuciferine could enhance the expression of PPARα mRNA;salvianolic acid B could enhance the expression of the HL mRNA;salvianolic acid B, curcumine, nuciferine and gold theragan base could enhance the expression of the ApoB100 mRNA;curcumine gold theragan could enhance the expression of the ApoAII mRNA;salvianolic acid B and gold theragan base could enhance the expression of the ApoCII mRNA. [Conclusion] Shugandaozhuo medicines have effects on fatty liver by increasing the expression of lipid-related genes.