手十二井穴刺络放血对一氧化碳中毒小鼠去甲肾上腺素含量和细胞色素氧化酶活性的影响*

Effect of stabbing collaterals bloodletting of the hand twelve well points on the content of NE and cytochrome oxidase activity in mice after carbon monoxide poisoning

关键词:  手十二井穴  刺络放血  一氧化碳中毒

Key words:  twelve-well point of the hand  stabbing collaterals bloodletting  carbon monoxide poisoning

摘要:

[目的]观察手十二井穴刺络放血对煤气中毒小鼠的促醒作用,并探讨其部分作用机制。[方法]选用成年雄性昆明小鼠共54只,随机分为正常组、模型组和治疗组,每组18只。模型组和治疗组小鼠分批在罐内静式吸入含一氧化碳(CO)浓度为4 300 ppm的空气60~70 min,正常组小鼠不给予CO气体,置于空罐中,时长同模型组。小鼠从罐中取出后,治疗组进行手十二井穴刺络放血。观察染毒后即刻、1 h和4 h小鼠静脉血中去甲肾上腺素含量,染毒后4 h脑组织中细胞色素氧化酶活性检测。[结果]治疗组与模型组相比,染毒后即刻、1 h和4 h,治疗组血液中去甲肾上腺素含量明显低于模型组;染毒后4 h,治疗组小鼠脑组织中细胞色素氧化酶活性明显高于模型组。[结论]手十二井穴刺络放血可以降低血液中去甲肾上腺素含量、提高脑组织中细胞色素氧化酶活性。

Abstract:

[Objective] To observe the effect of stabbing collaterals bloodletting on hand twelve well points on awaking the coma mice after carbon monoxide poisoning and discuss the mechanism of the action.[Methods] Fifty-four Kunming male mice were randomly divided into normal group,model group and treatment group,18 mice in each group.The mice in model group and treatment group mice were inhaled at a standstill in the tank with CO at concentration of 4 300 ppm for 60~70 minutes.Normal mice were not given with carbon monoxide gas in the empty tank for the same time equal to model group.Then the mice were taken out of tank and treated with stabbing collaterals bloodletting of the hand twelve well points in mice in treatment group.The content of NE in blood was detected immediately,1 hour and 4 hours after exposure,and cytochrome oxidase activity in brain tissue 4 hours after that was detected.[Results] Compared with model group,the content of NE in blood immediately,1 hour and 4 hours after exposure in treatment group was significantly lower than that in the model group.After exposure for 4 hours,cytochrome oxidase activity in brain tissue in the treatment group was significantly higher than that in model group.[Conclusion] Stabbing collaterals bloodletting of the hand twelve well points can decrease the content of NE in blood and increase cytochrome oxidase activity in brain tissue.