| 摘要: |
| [目的]探讨三七活血有效成分人参皂苷Rg1对大鼠血小板聚集的影响。[方法]体外分离大鼠血小板,采用比浊法观察不同浓度Rg1(0.5、1.5、2.5、3、4 mmol/L,)对体外大鼠二磷酸腺苷(ADP)诱导的血小板聚集的抑制作用,以阳性药阿司匹林作为对照;采用酶联免疫(ELISA)法检测Rg1对大鼠血小板环磷腺苷(cAMP)的影响。[结果]0.5 mmol/L人参皂苷Rg1组对血小板聚集无明显影响;阿司匹林组及人参皂苷Rg1(4、3、2.5、1.5 mmol/L)组最大聚集率均明显小于阴性对照组(P<0.01),其抑制率分别为18.95%,59.04%,40.37%,29.77%,9.77%;不同浓度Rg1组对ADP诱导后血小板cAMP的含量与ADP组没有显著性差异。[结论]Rg1可明显抑制ADP诱导的血小板聚集,但其机制并不是通过升高血小板内cAMP含量而抑制血小板激活。 |
| 关键词: 人参皂苷Rg1 血小板 聚集 环磷腺苷 |
| DOI：10.11656/j.issn.1673-9043.2012.01.11 |
| 分类号: |
| 基金项目:* 教育部高等学校博士学科点专项科研基金(200800630001);天津市高等学校科技发展基金(20110208);中国博士后基金 |
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| The effect of rat platelet aggregation and platelet cyclic AMP metabolism by Ginsenoside Rg1 |
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HUA Shen-yu, QU Feng, CHEN Li-ping, KANG Li-yuan, ZHANG Bo-li
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Tianjin State Key Laboratory Medicine, Tianjin University of TCM, Tianjin 300193, Chinese
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| Abstract: |
| [Objective] To explore the effective components of ginsenoside Rg1 on rat platelet aggregation and cAMP.[Method] Using turbidimetry in different concentration of Rg1 (0.5、1.5、2.5、3、4 mmol/L) of rats in vitro induced inhibition of platelet aggregation,to positive drug aspirin was used as control;to study the content of cAMP on rat platelet by Rg1.[Results] 0.5 mmol/L ginseng saponins group Rg1 on platelet aggregation effect;aspirin group and ginsenoside Rg1 (4、3、2.5、1.5 mmol/L) group maximal aggregation ratio were significantly smaller than the negative control group (P<0.01),the inhibition rates were 18.95%、59.04%、40.37%、29.77%、9.77%;Rg1 of different concentration groups on ADP after induction of platelet cAMP content and ADP group were not significant difference.[Conclusion] Rg1 can inhibit platelet activation,but not by elevating the content of rat platelet. |
| Key words: Rg1 platelet aggragation cAMP |
