复方苦参注射液对子宫内膜癌HEC-1B细胞Bcl-2/Bax、P27表达的影响*

Effects of compound Kushen injection on expression of Bcl-2/Bax and P27 in human endometrial cancer HEC-1B cell

关键词:  复方苦参注射液(岩舒)  子宫内膜癌  抗肿瘤  细胞凋亡  实验研究

Key words:  compound Kushen injection  human endometrial cancer  anti-tumor  apoptosis  experimental study

摘要:

[目的]探讨复方苦参注射液(岩舒)对人子宫内膜癌HEC-1B细胞Bcl-2/Bax、P27基因表达的影响。[方法]采用四甲基偶氮唑蓝(MTT)法观察不同浓度复方苦参注射液及组方成分苦参注射液,白土苓注射液,顺铂注射液对人子宫内膜癌HEC-1B细胞增殖的影响;采用逆转录聚合酶链反应法(RT-PCR)检测复方苦参注射液对人子宫内膜癌HEC-1B细胞中Bcl-2/Bax、P27mRNA表达水平的影响。[结果]复方苦参注射液、苦参注射液均可抑制HEC-1B细胞增殖,并呈剂量依赖性,组间差异有统计学意义(P<0.05);复方苦参注射液、苦参注射液、顺铂注射液作用于HEC-1B细胞48h后,与空白对照组相比在mRNA表达水平Bcl-2降低,而Bax、P27表达升高,差异有统计学意义(P<0.05)。[结论]复方苦参注射液、苦参注射液可抑制人子宫内膜癌HEC-1B细胞增殖;复方苦参注射液及苦参注射液可明显抑制Bcl-2表达并促进Bax、P27表达,说明复方苦参注射液抑制子宫内膜癌HEC-1B细胞增殖的作用机制在于苦参对Bcl-2/Bax基因家族和P27基因的表达的影响。

Abstract:

[Objective] To explore the effects of compound Kushen injection (Yanshu) on Bcl-2/Bax,P27 expression in endometrial cancer HEC-1B cell.[Methods] Using MTT method the inhibition of HEC-1B cell proliferation treated by compound Kushen injection, Kushen injection, Baituling injection and DDP injection was detected. Using RT-PCR method their expression of Bcl-2/Bax and P27 was also detected.[Results] Compound Kushen injection and Kushen injection could inhibit the proliferation of HEC-1B cell and the effect was dosedependent. The expression of Bcl-2 gene was reduced significantly while Bax and P27 gene were risen on mRNA level treated for 48 h by compound Kushen injection, Kushen injection and DDPC injection (P<0.05).[Conclusion] Compound Kushen injection and Kushen injection can inhibit the expression of Bcl-2 and promote the expression of Bax and P27. Compound kushen injection and it's active ingredients Kushen can reduce Bcl-2 expression in HEC1B cell while rise Bax and P27 gene on mRNA level.