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| 盐酸小檗碱眼用固体脂质纳米粒的研究* |
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李佳玮1, 赵海南1, 郭秀君1, AggreyMO1, 刘志东1, 李楠1, 李琳1, 潘卫三2
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1.天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 现代中药发现与制剂技术教育部工程研究中心 300193;2.沈阳药科大学 110016
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| 摘要: |
| [目的]制备盐酸小檗碱固体脂质纳米粒。[方法]采用乳化蒸发低温固化法制备盐酸小檗碱纳米粒,采用离体角膜透过实验对其体外进行评价。[结果]制备的纳米粒的包封率为51.1%,平均粒径为(19±2)nm,zeta电位为-11.5mV,表观渗透系数为(1.46±0.45)×10-6cm/s,与对照组相比增加了16%,差异有统计学意义(P<0.05)。[结论]所用制备工艺简单,可用于制备盐酸小檗碱固体脂质纳米粒。 |
| 关键词: 盐酸小檗碱 固体脂质纳米粒 离体角膜透过 |
| DOI：10.11656/j.issn.1673-9043.2012.03.09 |
| 分类号: |
| 基金项目:*国家自然基金项目(81001645);中国博士后科学基金项目(20090460701) |
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| Preparation of ophthalmic solid lipid nanoparticles loading berberine hydrochloride |
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LI Jia-wei1, ZHAO Hai-nan1, GUO Xiu-jun1, Aggrey MO1, LIU Zhi-dong1, LI Nan1, LI Lin1, PAN Wei-san2
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1.Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of TCM, Tianjin 300193, China;2.Shenyang Pharmaceutical University, Shenyang 110016, China
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| Abstract: |
| [Objective] To prepare ophthalmic solid lipid nanoparticles loading berberine hydrochloride.[Methods] The solid lipid nanoparticles loading berberine hydrochloride were prepared by emulsification-ultrasonic method.The isolated cornea permeation test of the formulation was studied in rabbit.The permeated amount of berberine hydrochloride was determined by high performance liquid chromatography.[Results] The entrapment efficiencies of nanoparticle was 51.1%.The average partic1e size was(19±2)nm.The zeta potential was -11.5 mV.The transparent coefficient of the formulation was(1.46±0.45)×10-6 cm/s,increased for 16% when compared with control (P<0.05).[Conclusion] The preparation technique is simple and stable,which could be used to prepare the solid lipid nanoparticles loading berberine hydrochloride. |
| Key words: berberine hydrochloride solid lipid nanoparticle cornea permeation in vitro |
