银杏叶提取物对胎鼠皮质神经元细胞的影响

Effects of ginkgo biloba extract on fetal rat cortical neuronal cells

关键词:  银杏叶  胎鼠皮质神经元  细胞活性  细胞膜  细胞抗氧化性

Key words:  ginkgo biloba extract  fetal rat cortical neuronal cells  cell activity  cell membrane  cellular oxidation resistance

摘要:

[目的] 在正常条件和缺氧条件下研究银杏叶提取物对胎鼠皮质神经元细胞的影响。[方法] 在正常条件和缺氧条件下分别检测对照组和加药组的细胞活性值、细胞上清液中乳酸脱氢酶(LDH)含量、超氧化物歧化酶(SOD)含量和丙二醛(MDA)含量。[结果] 在正常培养下,加药组细胞活性高于对照组,差异有统计学意义;加药组的LDH值小于对照组,差异有统计学意义;加药组的SOD值和MDA值与对照组差异无统计学意义;在缺氧条件下,加药组细胞活性高于对照组,差异有统计学意义;加药组的LDH值也高于对照组,差异有统计学意义;加药组的SOD值和MDA值均高于对照组,差异有统计学意义。[结论] 在正常培养条件下,本实验所选银杏叶提取物可以促进神经元细胞生长,降低细胞膜损伤,对细胞抗氧化性无显着影响;在缺氧培养条件下,本实验所选银杏叶提取物可以促进神经元细胞生长,增加细胞膜损伤,可以提高细胞抗氧化性。

Abstract:

[Objective] To study the effects of ginkgo biloba extract on fetal rat cortical neuronal cells under normal and hypoxic cell cultivations. [Methods] Under normal and hypoxic cultivations, test the control and dosing groups separately, for the cell activity values, and Lactate Dehydrogenase (LDH), Superoxide Dismutase (SOD), and Malondialdegyde (MDA) values in the cellular supernatant. [Results] Under normal cell cultivation, the cell activity values of the dosing group were higher than those of the control group; the differences were statistically significant. The LDH values of the dosing group were lower than those of the control group; the differences were statistically significant. The SOD and MDA values of the dosing group were different from those of the control group; the differences were not statistically meaningful. Under hypoxic cell cultivation, the cell activity values of the dosing group were higher than those of the control group; the differences were statistically significant. The LDH values of the dosing group were higher than those of the control group; the differences were statistically significant. The SOD and MDA values of the dosing group were higher than those of the control group; the differences were statistically significant. [Conclusion] Under normal cell cultivation, the ginkgo biloba extract can stimulate the cortical neuronal cells' growth, reduce cell membrane damage, and has no notable effects on cellular oxidation resistance. Under hypoxic cell cultivation, the ginkgo biloba extract can stimulate the cortical neuronal cells' growth, increase cell membrane damage, and increase cellular oxidation resistance.