| 摘要: |
| [目的] 研究石榴皮多酚对人乳腺癌细胞MDA-MB-231增殖及凋亡的影响。[方法] 四甲基偶氮唑盐(MTT)法检测石榴皮多酚对人乳腺癌MDA-MB-231细胞增殖抑制作用;流式细胞仪分析细胞周期及细胞凋亡。[结果] 石榴皮多酚对人乳腺癌细胞MDA-MB-231的增殖有抑制作用,且呈现浓度和时间依赖性;石榴皮多酚能诱导MDA-MB-231细胞凋亡,使癌细胞周期被阻滞在G2-M期。[结论] 石榴皮多酚可抑制人乳腺癌细胞MDA-MB-231增殖,诱导癌细胞凋亡,并阻滞癌细胞在G2-M期。 |
| 关键词: 石榴皮多酚 乳腺癌 凋亡 MDA-MB-231细胞 |
| DOI：10.11656/j.issn.1673-9043.2012.04.08 |
| 分类号: |
| 基金项目:江苏省自然科学基金重点项目(BK2002201). |
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| Effects of extract of pomegranate peel on proliferation and apoptosis of breast cancer cell line MDA-MB-231 |
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ZHANG Xun1, WU Ai-ping2, ZHANG Yong-hong2
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1.Academy of TCM of Jiangsu Province, The Hospital of Integrated Chinese and Western Medicine, Nanjing 210028, China;2.The First Clinical Medical College of Nanjing University of TCM, Jiangsu Province Hospital of TCM, Nanjing 210029, China
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| Abstract: |
| [Objective] To investigate the effects of extract of pomegranate peel on proliferation and apoptosis of breast cancer cell line MDA-MB-231. [Methods] MTT assay was used to measure the anti-proliferation effect of extract of pomegranate peel in breast cancer line MDA-MB-231. The apoptosis and the cell cycle were analyzed by flow cytometry. [Results] The extract could inhibit the proliferation of breast cancer cell line MDA-MB-231 in dose-and time-dependent manner. It could induce the apoptosis of MDA-MB-231 cells. The growth of cancer cells was arrested in G2-M phase. [Conclusion] Extract of pomegranate peel can inhibit the proliferation of breast cancer cell line MDA-MB-231, induce apoptosis and block the growth of cancer cells in G2-M phase. |
| Key words: extract of pomegranate peel breast cancer apoptosis MDA-MB-231 cells |
