D-半乳糖剂量及小鼠性别对衰老模型的影响

Impact of D-galactose’s dose and gender of mice on aging model

关键词:  D-半乳糖  衰老  学习记忆能力  性别  生长因子

Key words:  D-galactose  aging  learning and memory ability  gender  growth factor

摘要:

[目的] 考察D-半乳糖剂量以及实验小鼠性别对衰老模型形成的影响。[方法] 实验选用不同性别的ICR小鼠,分别采用随机数字表法分为正常对照组、D-半乳糖低(100 mg/kg)、中(300 mg/kg)、高 (1 000 mg/kg)剂量组。每日将D-半乳糖于颈背部皮下注射,对照组注射等量生理盐水,连续注射10周,采用Morris水迷宫考察模型小鼠学习记忆能力,取血检测血中生长因子及氧化相关指标,取脑组织进行苏木-伊红(HE)染色,考察脑组织病理形态变化。[结果] 与正常对照组比较,不同剂量D-半乳糖对小鼠学习记忆能力均有不同程度的损伤,但雌雄小鼠无明显差别,尤其高剂量组平均潜伏时间明显延长(P<0.05);D-半乳糖中、高剂量组小鼠血中血管内皮生长因子(VEGF)、胰岛素样生长因子-1(IGF-1)均呈不同程度降低(P<0.05或P<0.01),雌雄间无明显差异;D-半乳糖中、高剂量组小鼠血中丙二醛(MDA)浓度较正常对照组明显升高(P<0.05),超氧化物歧化酶(SOD)活力较正常对照组明显下降(P<0.05或P<0.01).[结论] ICR小鼠皮下注射300~1 000 mg/kg剂量的D-半乳糖连续10周可以不同程度的引起小鼠学习记忆能力明显下降,并且可能与氧化反应升高和血中生长因子水平降低有关,实验小鼠性别对D-半乳糖致衰老模型无明显影响。

Abstract:

[Objective] To observe the effects of D-galactose's dose and sex of mice on the aging model. [Methods] ICR mice with different gender were randomly divided into normal control group, low dose of D-galactose (100 mg/kg) group, middle dose(300 mg/kg) group, high dose(1 000 mg/kg). D-galactose was injected subcutaneously into the back of the neck daily and control group was injected with same amount of normal saline continuously for 10 weeks. Morris water maze was taken to observe the learning and memory ability. Blood sample was obtained to test the growth factors level and related parameters of oxidation. Brain tissue was harvested to observe the pathological morphological changes after HE staining. [Results] Compared with normal group, different dose of D-galactose could damage the learning and memory ability of mouse in different degrees, but no significant difference was observed between male and female mouse. The high dose group significantly extend the average latent period(P<0.05); Serum VEGF and IGF-1 in middle and high dose group were lower than control group with varying degrees (P<0.05 or P<0.01), No significant difference was observed between male and female; MDA concentrations of middle and high dose group were increased significantly when compared with normal group (P<0.05); SOD activity was significantly lower than normal group (P<0.05 or P<0.01). [Conclusion] ICR mouse subcutaneously received 300~1 000 mg/kg of D-galactose for 10 weeks could continuously decrease the learning and memory ability with different degrees obviously. This may be related to the increasing of oxidizing reaction and the decreasing of growth factor in serum. The mouse' gender had no obvious effect on aging model induced by D-galactose.