| 摘要: |
| [目的] 观察不同强度的永磁磁场对正常和缺氧条件下人脑微血管内皮细胞（HBMEC）增殖的影响。[方法] 采用正常和缺氧两种条件体外培养HBMEC，实验分为10组，即对照组、药物组（银杏叶注射液350 mg/L）、不同强度磁场组（3.4、5.6、13.8、27.8、52.6、107.0、178.9、292.2 mT），各组细胞于磁场作用48 h后收集样本，用四甲基偶氮唑盐法（MTT法）检测细胞增殖。[结果] 正常和缺氧条件下，与对照组相比较，药物组有统计学意义（P<0.05），各磁场组差异均无统计学意义（P>0.05）；磁场组组间比较，差异无统计学意义（P>0.05）.[结论] 正常和缺氧条件下，磁感强度3.4、5.6、13.8、27.8、52.6、107.0、178.9、292.2 mT的磁场对人脑微血管内皮细胞的增殖无影响。 |
| 关键词: 永磁磁场 人脑微血管内皮细胞 细胞增殖 |
| DOI：10.11656/j.issn.1673-9043.2013.04.06 |
| 分类号: |
| 基金项目:基金项目：国家自然科学基金资助项目（30873467） |
|
| Effect of magnetic fields on human brain microvascular endothelial cell proliferation under normal and hypoxia condition |
|
MENG Qing-nan1, WANG Yun-hua2, ZHANG Hao-nan1, YANG Lin2, WANG Yi-min1
|
|
1.Traditional Chinese Medicine Engineering Institute of Tianjin University of TCM, Tianjin 300193, China;2.Tianjin University of TCM, Tianjin 300193, China
|
| Abstract: |
| [Objective] To observe effect of different permanent magnetic fields on human brain microvascular endothelial cell（HBMEC） proliferation under normal and hypoxia conditions.[Methods] HBMEC were cultured in vitro under normal condition and hypoxia condition, experimental cells were divided into 10 groups, control group, ginkgo biloba injection（350 mg/L） group, different induction magnetic field groups （3.4, 5.6, 13.8, 27.8, 52.6, 107.0, 178.9, 292.2 mT）, each group was collected specimens 48 h after planking, MTT method was used to measure cell proliferation. [Results] Compared with control group, the drug group was statistically different（P<0.05） under normal condition and hypoxia condition, magnetic field groups were no statistically different（P>0.05）; there was no statistical difference between magnetic field groups（P>0.05）.[Conclusion] Magnetic field groups with magnetic induction 3.4,5.6,13.8,27.8,52.6,107.0,178.9,292.2 mT have no effect on proliferation of HBMEC cultured under normal or hypoxia condition. |
| Key words: permanent magnetic field human brain microvascular endothelial cell cell proliferation |
