昆布提取物对小鼠免疫功能及抗疲劳能力的影响

Influences of aqueous extract of kelp on immune function and anti-fatigue in mice

关键词:  昆布  免疫功能  抗疲劳  小鼠

Key words:  aqueous extract of Kelp  immune function  anti-fatigue effect  mice

摘要:

[目的] 观察昆布提取物（AEK）对小鼠免疫功能及抗疲劳能力的影响。[方法] 昆明种小鼠，体质量18~22 g，40只，分为4组，分别为AEK小剂量组（21.2 g/kg）、AEK大剂量组（106 g/kg）、生理盐水空白对照组和阳性对照甲状腺素组（20 mg/kg）.灌胃给药10 d后，分别测定AEK对小鼠腹腔巨噬细胞吞噬功能和淋巴细胞转化功能的影响，以及对二硝基氯苯（DNCB）致耳肿胀和抗疲劳能力的影响。[结果] AEK可提高小鼠腹腔吞噬细胞的吞噬百分率及吞噬指数；促进植物凝血素（PHA）诱导的淋巴细胞转化；促进DNCB引起的皮肤迟发超敏反应及溶血素抗体的生成；延长小鼠在水中游泳时间。[结论] AEK对小鼠非特异性免疫、细胞免疫及体液免疫均有增强作用，并能提高小白鼠的抗疲劳能力。

Abstract:

[Objective] To observe the influences of aqueous extract of Kelp（AEK） on immune function and anti-fatigue in Mice.[Methods] Mice（18～22） g were divided into 4 groups: low dose group（AEK 21.2 g/kg）, high dose group （AEK 106 g/kg）, blank control group（0.9% NaCl）, positive control group（thyroxine 20 mg/kg）. Drugs were administered to mice by i.g. for 10 days. The phagocytic activity of peritoneal macrophages, transformation of lymphocyte, the production of serum haemolysis and skin delayed-type hypersensitivity reaction induced by DNCB were determined. Anti-fatigue effect was evaluated by the swimming time in water. [Results] AEK could improve phagocytic activity of peritoneal macrophages and transformation of lymphocyte, and promote mouse skin delayed-type hypersensitivity reaction induced by DNCB as well as increase production of the haemolysis. Swimming time was prolonged by AEK. [Conclusion] Non-specific, cellular and humoral immunity is promoted, and anti-fatigue effect is increased by AEK in mice.