黄芩素PLGA纳米粒的体外评价及细胞相容性研究

In vitro evaluation and cellular biocompatibility of Scutellarein-loaded PLGA nanoparticle

关键词:  缓释  PLGA纳米粒  黄芩素  细胞相容性

Key words:  controlled release  PLGA nanoparticles  Scutellarein  biocompatibility

摘要:

[目的] 制备黄芩素聚（乳酸-羟基乙酸）共聚物（PLGA） 纳米粒，并对其理化性质、体外释药以及体外角膜细胞相容性进行研究. [方法] 使用乳化溶剂挥发法制备黄芩素PLGA纳米粒，评价其性质和体外缓释效果，主要包括：纳米粒粒径，纳米粒包封率，药物载药量和体外缓释曲线等. 采用细胞增殖实验评价黄芩素PLGA纳米粒的细胞毒性. [结果] 黄芩素PLGA纳米粒粒径（92.5±2.35） nm、Zeta电位（-21.1±2.5） mV、包封率（92.5±2.35）%、载药量（23.12±1.45）%.体外缓释实验提示：突释阶段黄芩素释放率在1 d内达（8.37±0.31）%，缓释阶段纳米粒可稳定释放，在10 d时释放达（51.30±0.50）%，细胞增殖实验提示黄芩素PLGA纳米粒对细胞体外生长无不良影响，细胞相容性好. [结论] 采用乳化溶剂挥发法制备的黄芩素PLGA纳米粒具有良好的缓释效应和良好的细胞相容性.

Abstract:

[Objective] To prepare Scutellarein loaded PLGA nanoparticles and characterize the physicochemical properties, in vitro release behavior and biocompatibility of HCEpic cells. [Methods] The PLGA nanoparticles were prepared by emulsion solvent diffusion method. The characterization and release effect of PLGA NPs in vitro were evaluated. The main outcome measures included size, Zeta potential, Encapsulation efficiency, the drug content of the final nanoparticles etc. The in vitro release curve was drawn. The cytotoxicity of PLGA NPs were evaluated by cell proliferation assay. [Results] The particle sizes, Zeta potential, Encapsulation efficiency and the drug content of the final nanoparticles were 128.4 nm, -21.1 mV, (92.5±2.35)% and (23.12±1.45)%, respectively. The release of Scutellarein from PLGA nanoparticles were markedly reduced compared with Scutellarein solution. Cell proliferation assay revealed the PLGA-NPs did not damage the cell growth in vitro, indicating a good compatibility. [Conclusion] The PLGA-NPs prepared by emulsion solvent diffusion method have a good release effect and good biocompatibility in vitro.