“半蒌贝蔹及攻乌”反药组合对制川乌镇痛作用的影响

Impact of contrary medicine combination on analgesia effect of processed Aconiti Radix before and after combination with Pinellia, Snakegourd Fruit, Fritillary, Ampelopsis Japonica Makino and Rhizoma Bletillae

关键词:  制川乌  配伍  甩尾法  镇痛

Key words:  Processed Aconite Radix  compatibility  Tail-Flick method  analgesia

摘要:

[目的] 考察“半蒌贝蔹及攻乌”反药组合对制川乌镇痛效果的影响. [方法] 采用辐射热刺激甩尾法检测给药前及给药后不同时间点小鼠的痛阈值，明确“半蒌贝蔹及攻乌”各单味药的镇痛效果；在此基础上，将制川乌与各单药以1：1配伍，考察反药组合对制川乌镇痛效果的影响. [结果] 制川乌在含生药4 g/kg剂量下，15 min时镇痛效果最佳，与给药前相比差异有统计学意义；法半夏、瓜蒌、白及单独使用有一定的镇痛作用，与给药前相比差异有统计学意义，但起效时间晚于制川乌；川贝母、白蔹镇痛作用与给药前相比差异无统计学意义；法半夏、川贝母、白及与制川乌配伍后可使制川乌的镇痛起效时间延后；瓜蒌与制川乌配伍使镇痛作用起效时间提前，并可持续一定时间（约30 min）；白蔹与制川乌配伍后可显着降低制川乌的镇痛效果. [结论] 降低乌头的镇痛作用或延迟起效时间可能是“半蒌贝蔹及攻乌”反药组合相反的具体表现之一.

Abstract:

[Objective] To study the impact on analgesia of processed Aconiti Radix analgesia before and after combination with Pinellia, Snakegourd Fruit, Fritillary, Ampelopsis Japonica Makino and Rhizoma Bletillae. [Methods] Using the tail-flick method by radiant heat stimulation the pain threshold of the mice was detected at different time before and after oral administration. The analgesic effect of every single herb was cleared. On this basis, the Processed Aconite Radix was combined with every single herb at 1:1 and then the impact of anti-drug combination on Processed Aconite Radix analgesic effect was detected. [Results] Processed Aconite Radix could significantly increase the pain threshold in mice at 15 min compared with that before administration under the dose of 4 g crude drug/kg; Pinellia, Snakegourd Fruit and Rhizoma Bletillae used alone had some analgesic effect. The difference was significant compared with that before administration, but the onset time was more delayed than Processed Aconite Radix; For Fritillaria, Ampelopsis Japonica Makino in analgesic effect. There was no significant difference compared with that before administration. Pinellia, Fritillaria and Rhizoma Bletillae could delay the onset time of Processed Aconite Radix and Snakegourd Fruit could make the onset time in advanced and sustainable for a certain period of time (about 30 min). Ampelopsis Japonica could significantly reduce the analgesic effect of Processed Aconite Radix. [Conclusion] Reducing the analgesic effect of Aconitum or delaying the onset time may be one of the specific performance of processed Aconite Radix after combination with Pinellia, Snakegourd Fruit, Fritillary, Ampelopsis Japonica Makino and Rhizoma Bletillae.