芍药苷ApoE-/-对小鼠血脂及主动脉斑块的影响

Effect of Paeoniflorin on blood lipids and atherosclerotic plaque in mice with apolipoprotein E deficient

关键词:  芍药苷  ApoE^-/-小鼠  高脂血症  动脉粥样硬化

Key words:  Paeoniflorin  ApoE^-/- mice  hyperlipidemia  atherosclerosis

摘要:

[目的]观察芍药苷对ApoE^-/-小鼠血脂及主动脉斑块形成的影响。[方法]6周龄ApoE^-/-雄性小鼠经普通饲料饲养4周，髙脂饲料饲养12周后，随机分为ApoE^-/-模型组及芍药苷60 mg/kg、30 mg/kg剂量组，相同遗传背景的同龄正常C57BL/6J小鼠作为空白对照组。药物干预12周后，检测血脂的变化，并测量主动脉弓部、胸段、腹段斑块面积。[结果]ApoE^-/-模型组甘油三酯（TG）、总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）显着高于C57BL/6J组（P<0.01），芍药苷60 mg/kg、30 mg/kg组的血脂水平显着低于ApoE^-/-模型组（P>0.05，P<0.01），并且不同程度减小主动脉各段斑块的面积。[结论]芍药苷具有降脂和抑制主动脉斑块形成的作用，表明芍药苷具有抗动脉粥样硬化作用。

Abstract:

[Objective] To observe the effect of paeoniflorin on ApoE^-/- mice blood lipids and the formation of atherosclerosis.[Methods] Six week old ApoE^-/- male mice were normal diet feeding four weeks, high fat diet after 12 weeks were randomly divided into three groups: ApoE^-/- model group and paeoniflorin 60 mg/kg, 30 mg/kg dose groups, the same genetic background of normal C57BL/6J mice of the same age as the control group. After 12 weeks of paeoniflorin intervention, observed the changes in serum lipids and measure the plaque area of arch department, chest and abdomen section of aorta.[Results] Compared with C57BL/6J group,ApoE^-/- model triglyceride (TG), total cholesterol(TC) and low density lipoprotein cholesterol (LDL) were significantly increased (P<0.01), while the paeoniflorin 60 mg/kg, 30 mg/kg groups serum lipid levels were significantly reduced than the ApoE^-/- model group (P>0.05, P<0.01), and varying degrees of diminish the aortic plaques area in each segment.[Conclusion] Paeoniflorin has the effect of decreasing the level of blood lipids, and inhibiting the formation of aortic plaques, indicating that paeoniflorin has the effect of anti-atherosclerosis.