和营安心方对胚胎心肌细胞氧化应激性损伤的影响

Effect of Heying Anxin prescription on oxidative stress injury in embryo myocardium of rats

关键词:  和营安心方  过氧化氢  心肌细胞  氧化应激

Key words:  Heying Anxin prescription  H₂O₂  myocardium cell  oxidative stress

摘要:

[目的]研究和营安心方对过氧化氢（H₂O₂）所致大鼠胚胎心肌细胞氧化应激损伤的影响。[方法]用70%乙醇冷凝回流提取和营安心方，采用H₂O₂建立大鼠胚胎心肌细胞氧化应激损伤模型。实验分为5组，分别为：正常对照组、H₂O₂（200 μmol/L）模型组、和营安心方高、中、低剂量组，其中和营安心方高、中、低剂量组分别加入和营安心方提取物500、250、125 mg/kg ，24 h之后加入与模型组同浓度的H₂O₂（200 μmol/L），共同作用2 h.观察心肌细胞四甲基偶氮唑盐（MTT）代谢率、乳酸脱氢酶（LDH）含量、丙二醛（MDA）含量及各组细胞培养液中过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的含量。[结果]H₂O₂组MTT代谢率显着性降低（P<0.01），LDH及MDA含量显着性升高（P<0.01），CAT及SOD含量显着性降低（P>0.05）；和营安心方可使H₂O₂损伤的心肌细胞MTT 代谢率提高，MDA 含量减少（P>0.05），LDH 活性降低（P>0.05），并且显着性提高心肌细胞的CAT和SOD含量（P>0.05）.[结论]和营安心方对受损伤的心肌细胞具有明显的保护作用，其机制可能与抑制氧化损伤有关。

Abstract:

[Objective] To investigate the effect of Heying Anxin prescription on embryo myocardium injury caused by H₂O₂.[Methods] Reflux condenser extraction with 70% ethanol of Heying Anxin prescription was used and the cardiomyocytes were incubated with H₂O₂(200 μmol/L) for 2 hours in model group. the same dose of H₂O₂ was administered for 2 hours after treatment with Heying Anxin prescription (500 mg/kg, 250 mg/kg, 125 mg/kg) in therapeutic group and incubated for another 24 hours. Metabolic rate of MTT, content of MDA, activity of LDH and the levels of CAT and SOD in each group were measured.[Results] Metabolic rate of MTT: compared with control group, metabolic rates of MTT was decreased significantly in H₂O₂ group(P<0.01), while Heying Anxin prescription had no significant effect on cardiac myocytes of MTT metabolic rates. Content of MDA: compared with that of H₂O₂ group, the content of MDA was decreased in Heying Anxin prescription groups significantly(P>0.05).Content of LDH: compared with that of control group, the content of LDH was decreased in Heying Anxin prescription groups significantly(P>0.05).Content of CAT: compared with that of H₂O₂ group, the content of CAT was elevated in Heying Anxin prescription groups significantly(P>0.05).Content of SOD:compared with that of H₂O₂ group,the content of SOD was elevated in Heying Anxin prescription groups significantly(P<0.01).[Conclusion] Heying Anxin prescription has significant protective effects on injured myocardium cell induced by H₂O₂ in rats, and it may be related to the inhibition of oxidative damage.