金银花抗氧化活性整合指纹图谱的建立及其在质量评价中应用

Establishment of the antioxidant activity integrated fingerprint of Flos Lonicerae and its application in quality evaluation

关键词:  金银花  抗氧化活性指纹图谱  HPLC-DAD-DPPH甲醇枸橼酸在线体系

Key words:  Flos Lonicerae  antioxidant activity integrated fingerprint  HPLC-DAD-DPPH methanol citrate online system

摘要:

[目的] 建立评价金银花抗氧化活性的方法，比较不同产地金银花药材抗氧化活性大小，确定抗氧化活性成分的贡献率。[方法] 采用在线快速活性筛选高效液相色谱-二极管阵列检测器-1，1-二苯基-2-苦基肼（HPLC-DAD-DPPH）甲醇枸橼酸在线体系，建立金银花抗氧化活性指纹图谱。[结果] 确定了绿原酸和异绿原酸A为其主要的抗氧化活性成分，以金银花中8个主要活性成分为抗氧化活性指标对不同产地金银花的质量进行控制，确定了广州野生金银花质量最佳。[结论] 该方法简便可行，基于活性整合指纹图谱技术筛选药材中的抗氧化活性成分并以活性成分为指标，为全面有效评价中药质量提供了可借鉴的模式。

Abstract:

[Objective] To establish a method for evaluating the antioxidant activity of Flos Lonicerae, comparing antioxidant activity of Flos Lonicerae from different origins and determine the contribution rate of the each active antioxidant ingredient.[Methods] Using an active filter with fast online system, HPLC-DAD-1,1-diphenyl-2-picrylhydrazyl (HPLC-DAD-DPPH)in methanol citrate online system, the antioxidant activity fingerprints of Flos Lonicerae was established. [Results] Identifying the main antioxidant active ingredients and using chlorogenic acid and chlorogenic acid A, the quality control of Flos Lonicerae in different origins was accomplished taking eight main active ingredients in Flos Lonicerae as the indicators of antioxidant activity. It was demonstrated that the quality of the wild Flos Lonicerae from Guangzhou was the best amang all selected samples.[Conclusion] This method is simple and feasible. It is based on activity integrated fingerprint technology for screening the active antioxidant ingredients in herb and as an index, providing a reference model for evaluation of the quality of traditional Chinese medicine comprehensively and effectively.