酚-黄复方对痤疮致病菌的体外抗菌作用研究

External anti-bacterial effects of bakuchiol and baicalin on pathogenic bacteria of acne

关键词:  补骨脂酚  黄芩苷  痤疮丙酸杆菌  表皮葡萄球菌  菌落计数法  抑菌作用

Key words:  bakuchiol  baicalin  staphylococcus epidermidis  propionibacterium acnes  colony counting method  anti-bacterial effect

摘要:

[目的] 通过体外抑菌实验，考察补骨脂酚与黄芩苷组合用药的体外抗痤疮菌活性。[方法] 采用菌落计数法，以不同浓度补骨脂酚及加入黄芩苷后对表皮葡萄球菌和痤疮丙酸杆菌生长的抑菌率为指标考察其抑菌效果。[结果] 当补骨脂酚浓度达到2 560 mg/L时对表皮葡萄球菌及痤疮丙酸杆菌的抑菌率分别为100.0%、61.1%.在补骨脂酚溶液中加入黄芩苷后两药浓度分别为2 560 mg/L和800 mg/L，对两种痤疮菌的抑菌率均为100.0%，在此浓度下两药合用对痤疮丙酸杆菌的抑制作用更为明显，抑菌率是单用补骨脂酚的1.64倍。[结论] 酚-黄复方对痤疮菌的抑菌率增加显着，表明酚-黄复方共同发挥抗痤疮菌作用，揭示酚-黄合用的必要性和合理性。

Abstract:

[Objective] To study tha external anti-bacterial activity of bakuchiol and baicalin in acne.[Methods] The colony counting method was applied to determine the inhibitory ratio against staphylococcus epidermidis and propioni-bacterium acnes growth by adding the mixed solution with different concentrations of bakuchiol and bakuchiol-baicalin. [Results] The inhibitory ratios against staphylococcus epidermidis and propionibacterium acnes growth were 100.0% and 61.1% with 2 560 mg/L bakuchiol solution. Two kinds of acne bacteria inhibitory rate were 100.0% under the mixed solutions with the bakuchiol concentration of 2 560 mg/L and baicalin 800 mg/L. In particular, the propionibacterium acnes inhibitory rate was improved for 1.64 times than single bakuchiol.[Conclusion] The increasing of the inhbitory rate in combination of a certain amount of bakuchiol and baicalin indicates that they can perform a synergism function,which can significantly enhance the anti-acne bacterial effects,revealing the necessity and rationality on the inhibition against staphylococcus epidermidis and propionibacterium acnes growth.