| 摘要: |
| [目的] 检测三甲散含药血清对肝星状细胞株(HSC-T6)细胞中α-平滑肌肌动蛋白(α-SMA)、基质金属蛋白酶抑制-1(TIMP-1)和蛋白激酶(ROCK-Ⅰ)的表达,从而研究三甲散对HSC-T6细胞增殖作用的影响。[方法] 以三甲散含药血清干预HSC-T6细胞,采用实时荧光定量聚合酶链式反应(RT-PCR)和免疫印迹(Western blot)技术,检测三甲散含药血清对HSC-T6细胞中α-SMA、TIMP-1和ROCK-Ⅰ的蛋白表达。[结果] 三甲散和Y- 27632显著地降低了HSC-T6细胞α-SMA、ROCK-Ⅰ和TIMP-1 mRNA和蛋白的表达。[结论] 三甲散可降低HSC- T6细胞ROCK-Ⅰ蛋白、TIMP-1和α-SMA的表达,从而抑制HSC-T6细胞的增殖。 |
| 关键词: 三甲散 α-SMA TIMP-1 ROCK-Ⅰ 蛋白表达 |
| DOI：10.11656/j.issn.1673-9043.2014.06.11 |
| 分类号: |
| 基金项目:陕西省科技厅资助项目(2013JQ4007)。 |
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| Effect of Sanjia powder on expression of protein of α-SMA, TIMP-1, ROCK-Ⅰ in hepatic stellate cell |
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ZHENG Xu-rui, HUI Yi, YAN Shu-guang, SONG Xiao, CAO Ning, WANG Li-feng, CHAO Xu
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Shanxi University of Traditional Chinese Medicine, Xianyang 712046, China
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| Abstract: |
| [Objective] The expression of α-SMA, TIMP-1 and ROCKⅠ in the hepatic(HSC-T6) stellate cell were measured to study the proliferating effect of Sanjia powder. [Methods] HSC-T6 cells interfered by Sanjia powder-containing serum, RT-PCR and Western blot were used to detect the expression of α-SMA, TIMP-1, ROCKⅠ in the hepatic stellate cell. [Results] Real-time PCR and Western blot assay showed that Sanjia powder and Y-27632 could reduce the expression of ROCK-Ⅰprotein, TIMP-1 and a-SMA mRNA in HSC-T6 cells. [Conclusion] Sanjia powder can decrease the expression of ROCK-Ⅰ protein, TIMP-1 and a - SMA in HSC-T6 cells. |
| Key words: Sanjia powder α-SMA TIMP-1 ROCK-Ⅰ protein expression |
