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| UPLC法测定痤疮凝胶中黄芩苷和蒙花苷的含量 |
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范丽丽1, 李楠1, 顾星1, 朱蕴1, 刘志东1, 夏庆梅2, 郭丽丽1, 王爱潮1
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1.天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;2.天津中医药大学, 天津 300193
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| 摘要: |
| [目的] 建立痤疮凝胶中黄芩苷、蒙花苷含量测定方法.[方法] 采用Acquity BEH C18(2.1 mm×50 mm,1.7 μm)色谱柱;以乙腈-0.1%甲酸(20:80)为流动相,流速0.3 mL/min,检测波长320 nm,柱温35 ℃. [结果] 黄芩苷和蒙花苷分别在3.767～75.34 μg和0.840 96～33.638 4 μg范围内线性关系良好,平均回收率分别为99.04%和97.42%(n=6);RSD分别为1.97%和1.81%.[结论] 该方法简便、准确、重现性良好,为痤疮凝胶的质量控制提供了理论依据. |
| 关键词: 痤疮凝胶 黄芩苷 蒙花苷 超高效液相色谱法 含量测定 |
| DOI：10.11656/j.issn.1673-9043.2015.01.12 |
| 分类号: |
| 基金项目:教育部新世纪优秀人才支持计划(NCET-12-1068). |
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| Determination of Baicalin and Linarin in Cuochuang Gel by UPLC |
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FAN Li-li1, LI Nan1, GU Xing1, ZHU Yun1, LIU Zhi-dong1, XIA Qing-mei2, GUO Li-li1, WANG Ai-Chao1
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1.Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To establish a UPLC method for content determination of Baicalin and Linarin in Cuochuang Gel. [Methods] The column was Acquity BEH C18(2.1 mm×50 mm, 1.7 μm). The mobile phase consisted of a mixture acetonitrile-1%formic acid(20:80). The flow rate was 0.3 mL/min.The detection wavelength was 320 nm under 35 ℃. [Results] The calibration curves for baicalin and Linarin were linear in the ranges of 3.767~75.34 μg and 0.840 96~33.638 4 μg, and the average recovery was 99.04% and 97.42%(n=6), with RSD of 1.97% and 1.81%, respectively. [Conclusion] This method was simple, precise and repeatable, which can be used for quality control of Cuochuang Gel. |
| Key words: Cuochuang Gel baicalin Linarin UPLC content determination |
