| 摘要: |
| [目的] 对墨旱莲中的化学成分进行分离鉴定。[方法] 采用正相硅胶、反相ODS(十八烷基键合硅胶)、凝胶等柱色谱及制备型高效液相色谱(HPLC)法分离纯化, 并利用质谱分析(MS)、核磁共振波谱(NMR)等方法对化合物结构进行鉴定。[结果] 从墨旱莲体积分数为70%的乙醇提取物中分离鉴定了9个化合物, 分别鉴定为2,2';5', 2"-三联噻酚(1)、5-醛基-2,2';5', 2"-三联噻酚(2)、5-羟甲基-2,2';5', 2"-三联噻酚(3)、2,2';5', 2"-三联噻酚-5-羧酸(4)、5-醛基-5'-(3-丁烯-1-炔基)-2,2'-二联噻酚(5)和5-丁-1'-炔-3'-羟基-4'-氯-(2-戊-1", 3"二炔)-噻酚(6),二氢猕猴桃内酯(7),黑麦草内酯(8),原儿茶酸乙酯(9).[结论] 化合物6～9为首次从鳢肠属植物中分离得到。 |
| 关键词: 墨旱莲 化学成分 结构鉴定 |
| DOI：10.11656/j.issn.1673-9043.2014.11 |
| 分类号: |
| 基金项目:天津市高等学校科技发展基金一般资助项目(20120203);高等学校博士学科点专项科研基金(20121210110007). |
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| Isolation and identification of chemical constituents from Eclipta prostrata L. |
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MA Di1, HAN Li-feng2, LIU Er-wei2, XIA Ming-hui2, ZHANG Yi2, WANG Tao2
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1.First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China
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| Abstract: |
| [Objective] To isolate the chemical constituents of Eclipta prostrata L. and elucidate their structures. [Methods] The compounds were isolated by silica gel, ODS, Sephadex LH-20 column chromatography and PHPLC, and their structures identified by MS and NMR. [Results] Nine compounds were isolated from 70% ethanol extract of Eclipta prostrata L. and identified as 2, 2'; 5', 2''-terthiophene (1), α-formylterthienyl (2), 5-hydroxymethyl-2, 2'; 5', 2''-terthiophene (3), 2,2'; 5',2"-terthiophene-5-carboxylic acid (4), 5'-carboxaldehyde-5-(3-butene-1-ynyl)-2, 2'-bithiophene (5), 4'-chloro-1'-(5-penta-1,3-diyn-1-yl-2-thienyl)-but-2'-yn-3'-ol (6), dihydroactinidiolide (7), loliolide (8) and protocatechoic acid ethyl ester (9), respectively. [Conclusion] Compounds 6-9 are obtained from Eclipta prostrata for the first time. |
| Key words: Eclipta prostrata L. chemical constituents structure identification |
