| 摘要: |
| [目的] 观察心复康联合骨髓间充质干细胞(BMSC)调节心脏干细胞(CSC)内皮细胞标记物CD31(CD31)和血管内皮生长因子受体2(VEGFR2)的蛋白表达。[方法] 原代获取BMSC,制备并鉴定心复康含药血清,酶联免疫吸附法(ELISA法)检测心复康对BMSC分泌基质细胞衍生因子1α(SDF-1α)的影响。组织块贴壁法分离CSC,并行流式细胞分选及免疫荧光鉴定。利用Transwell小室建立细胞共培养体系,实验分为对照组、心复康组、抑制剂组,蛋白质印迹法(Western Blot)分析各组CSC的CD31和VEGFR2蛋白表达情况。[结果] 与另两组相比,心复康组CSC的CD31和VEGFR2蛋白表达显着增高,差异有统计学意义(P<0.05).[结论] BMSC经心复康干预后,可促进CSC的CD31和VEGFR2蛋白表达,提示SDF-1α/CXCR4轴为其作用机制之一。 |
| 关键词: 心复康 骨髓间充质干细胞 心脏干细胞 内皮细胞标记物CD31 血管内皮生长因子受体2 |
| DOI：10.11656/j.issn.1673-9043.2015.05.05 |
| 分类号: |
| 基金项目:国家自然科学基金项目(81173413). |
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| Effect of Xinfukang combined with Bone marrow mesenchymal stem cells on the expression of CD31 and VEGFR2 in cardiac stem cells |
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WANG Yi-jing1, ZENG Wen-yun1, LI Hu-hu1, WEI Bing1, KAN Bo-hong2, FAN Ying-chang1
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1.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To observe the regulation of CD31 and VEGFR2 protein expression in cardiac stem cells (CSC) under the the synergy between Xinfukang and bone marrow mesenchymal stem cells (BMSC). [Methods] The concentration of SDF-1α from BMSC with Xinfukang were detected by Elisa. The CSC was obtained through tissue explant method, and the purity was evaluated by flow cytometry and immunofluorescence. During the experiment, the CSC pretreated with AMD3100 were co-cultured with Xinfukang,72 h later, the protein expression of CD31 and VEGFR2 in CSC was detected by Western Blot. [Results] Compared with the other two groups, the CD31 and VEGFR2 protein expression of CSC in the Xinfukang group was significantly increased (P<0.05). [Conclusion] BMSC with Xinfukang can increase the protein expression of CD31 and VEGFR2 in CSC,and the mechanism may be related to SDF-1α/CXCR4 axis. |
| Key words: Xinfukang BMSC CSC CD31 VEGFR2 |
