黄地吉仙汤对2型糖尿病大鼠FBG及HbA1c影响的实验研究

Experiment study of Huangdi Jixian decoction on FBG and HbA1c in type 2 diabetes

关键词:  2型糖尿病  黄地吉仙汤  空腹血糖  糖化血红蛋白

Key words:  Type 2 diabetes  Huangdi Jixian decoction  FBG  HbA1c

摘要:

[目的] 观察“补泻同施,调节平衡”法指导组方的经验方黄地吉仙汤对2型糖尿病大鼠空腹血糖(FBG)、糖化血红蛋白(HbA1c)的影响。[方法] 将清洁级雄性SD大鼠60只,随机抽取10只设置为空白对照组,其余50只给予高脂高糖饲料加一次性小剂量腹腔注射链脲菌素(STZ)与精神紧张法诱发2型糖尿病大鼠模型。造模成功后随机分为模型组、西药治疗组、中药治疗低、中、高剂量组,每组10只。除空白对照组外,其余5组分别喂药观察8周,给药前后观察大鼠一般情况、测定体质量、FBG、HbA1c指标。[结果] 西药治疗组、中药高、中、低剂量组大鼠经给药治疗后,一般情况均有不同程度的改善,中药高剂量组体质量与其他4组比较明显升高(P<0.05);西药治疗组、中药高、中剂量组与模型组比较血糖明显减低(P<0.05);中药高剂量组具有明显降低HbA1c的作用。[结论] 黄地吉仙汤可以明显改善糖尿病大鼠的一般情况,对糖尿病大鼠的体质量减轻具有缓解作用,能显著降低血糖及HbA1c,有效治疗2型糖尿病。

Abstract:

[Objective] To observe the effects of Huangdi Jixian decoction guided by “reinforcing and reducing, adjustment the balance” method on the FBG and HbA1c in type 2 diabetic rats. [Methods] Sixty male SD rats of clean grade, randomly selected 10 rats as the normal control group, the remaining 50 of given high fat diet add disposable small dose of intraperitoneal injection of STZ add stress to induce rat model of type 2 diabetes mellitus. After the success of modeling, 50 rats were randomly divided into model group, western medicine group, Chinese medicine treatment of low, medium and high dose groups, 10 rats in each group. All groups were treated 8 weeks, before and after administration of the body weight, the determination of FPG, HbA1c were administered. [Results] Western medicine treatment group, Chinese herbal medicine in high, medium and low dose group rats were improved in general condition after treatment. The weight in the high dosage group of Chinese medicine increased significantly than those in the other four groups (P<0.05). The FBG in the western medicine treatment group, Chinese medicine of high, middle dose group were decreased significantly than model group (P<0.05 or 0.01). Traditional Chinese medicine of high dose can significantly reduced HbA1c.[Conclusion] Huangdi Jixian decoction can obviously improve the diabetic rats in general condition, significantly reduced blood glucose and HbA1c.