升清降浊方对MSG肥胖大鼠胰岛素增敏作用的影响

Effects of shengqing Jiangzhuo prescription to improve the insulin sensitivity of MSG rats

关键词:  升清降浊方  MSG肥胖大鼠  胰岛素抵抗

Key words:  Shengqing Jiangzhuo prescription  MSG rats  insulin resistance

摘要:

[目的] 研究升清降浊方(SQJZF)对L-谷氨酸钠(MSG)肥胖大鼠胰岛素增敏作用的影响及其机制研究。[方法] 复制MSG肥胖大鼠模型,随机分为模型对照组、阳性药罗格列酮组、阳性药非诺贝特组、SQJZF高剂量组(含生药6 g/kg)、SQJZF中剂量组(含生药3 g/kg)、SQJZF低剂量组(含生药1.5 g/kg),另取正常组大鼠8只。连续给药50 d后,分别检测大鼠血清瘦素和肌糖元含量;实时荧光定量聚合酶链式反应(PCR)检测药物对MSG大鼠骨骼肌过氧化物酶体增殖物激活受体 (PPAR )、瘦素(Leptin)、胰岛素受体底物-1(IRS-1)、胰岛素受体底物-2(IRS-2)、葡萄糖转运蛋白4(GLUT₄)的基因表达水平。[结果] 模型组MSG大鼠肌糖元和血清瘦素含量显著升高(P<0.01或P<0.05),相关基因PPAR 、Leptin、IRS-1、IRS-2、GLUT₄的相对表达显著降低(P<0.01);给药50 d后,各给药组肌糖元和血清胰岛素含量均有降低,对相关基因的相对表达均有一定的调节作用。[结论] 升清降浊方可能通过激活PPAR 受体,调控Leptin基因的表达,增加胰岛素的敏感性。

Abstract:

[Objective] To study the mechanisms and effects of Shengqing Jiangzhuo prescription to improve the insulin sensitivity of MSG rats. [Methods] Copy the MSG obese rat models, which were randomly divided into model group, rosiglitazone group, fenofibrate group, three dose of Shengqing Jiangzhuo prescription. Then choose 8 normal rats as the normal control group. Measure the levels of glucose in skeletal muscle and the level of leptin in serum after administration for 50 days. Real-time PCR detect the expression level of PPAR, leptin, IRS1, IRS2, GLUT₄ in skeletal muscle. [Results] Compared with the normal group, the model group improved the level of glucose in skeletal muscle and the level of leptin in serum significantly (P<0.01), and decreased the expression of PPAR, leptin, IRS1, IRS2, GLUT₄ significantly (P<0.01, P<0.05). Compared with the model group, each of groups had some improvements of the level of glucose in skeletal muscle and the level of leptin and the level of genes expression. [Conclusion] Shengqing Jiangzhuo prescription improve the insulin sensitivity in MSG rats which by activating the genes expression of PPAR and leptin.